Description

Mung Bean Nuclease CAS NO 54576-84-0 is a highly specific single-strand endonuclease derived from mung bean sprouts, prized for its ability to cleave single-stranded DNA and RNA while leaving double-stranded nucleic acids largely intact. This enzymatic specificity makes it an indispensable tool for molecular biology research and biotechnological applications. It is a critical reagent for researchers and manufacturers in the life sciences, molecular diagnostics, and pharmaceutical development sectors.

Application

• Removal of Single-Stranded Overhangs in DNA cloning and vector preparation.
• Site-Directed Mutagenesis for cleaving heteroduplex DNA containing mismatches.
• Mapping of Transcriptional Start Sites and intron-exon boundaries via S1 nuclease mapping assays.
• Analysis of Nucleic Acid Structure and hybridization, including probing for single-stranded regions.
• Cleaning Up PCR Products by digesting primer-dimer and other single-stranded artifacts.
• Next-Generation Sequencing (NGS) Library Preparation for specific processing steps.

Basic Information

Product Name	Mung Bean Nuclease
CAS No.	54576-84-0
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Mung Bean Nuclease; MBN; Single-Strand-Specific Nuclease; Nuclease S1 (from Aspergillus oryzae is a related enzyme); EC 3.1.30.1; Endonuclease S1; Single-Stranded Nucleic Acid Endonuclease; Mung Bean Sprout Nuclease
EINECS	Contact for details

Quality Control

Our Mung Bean Nuclease is produced and tested under stringent quality management systems. Each batch undergoes rigorous analytical testing to ensure high specific activity, purity, and absence of contaminating nucleases (e.g., double-strand specific nucleases, phosphatases). Certificates of Analysis (COA) detailing activity units/mg, purity by SDS-PAGE, and functional performance data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C in a non-frost-free freezer. Aliquot to avoid repeated freeze-thaw cycles. The product is supplied in a suitable buffer containing glycerol.

Specification

Item	Specification
Appearance	Clear to slightly opalescent liquid
Identification (Activity)	Cleaves single-stranded DNA/RNA
Specific Activity	> 20,000 units/mg protein
Purity (SDS-PAGE)	> 95%
Contaminating Nuclease Activity	Not detectable
Protein Concentration	Provided on COA
Storage Buffer	50 mM Tris-HCl, pH 8.0, 50% Glycerol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.