Description

Guanidine Thiocyanate is a powerful chaotropic salt essential for molecular biology and nucleic acid research. Its primary value lies in its ability to effectively denature proteins while stabilizing RNA, making it a critical component for high-yield, high-purity nucleic acid isolation. This reagent is indispensable for research laboratories, diagnostic kit manufacturers, and biopharmaceutical companies engaged in genomics, transcriptomics, and pathogen detection.

Application

• RNA Isolation & Purification: Key chaotropic agent in single-step RNA extraction methods (e.g., TRIzol™-like reagents) for tissues, cells, and blood.
• Viral Nucleic Acid Extraction: Used in lysis buffers for viral load testing and molecular diagnostics, including for HIV, HCV, and SARS-CoV-2.
• DNA Purification: Facilitates the purification of genomic DNA from complex biological samples by disrupting cellular structures.
• Protein Denaturation: Employed in studies requiring the denaturation or solubilization of proteins for analysis.
• In Vitro Transcription: Serves as a component in certain buffer systems to prevent RNase activity during mRNA synthesis.
• Laboratory Reagent Formulation: A raw material for manufacturing proprietary lysis buffers, hybridization solutions, and other molecular biology reagents.

Basic Information

Product Name	Guanidine Thiocyanate
CAS No.	593-84-0
Molecular Formula	CH5N3·CHNS
Molecular Weight	118.16 g/mol
Synonyms	Guanidinium Thiocyanate; Guanidine Isothiocyanate; GITC; GTC; Guanidine Sulfocyanate; Thiocyanic Acid Guanidine Salt; Guanidine Rhodanide; Guanidine, Thiocyanate
EINECS	209-812-1

Quality Control

Our Guanidine Thiocyanate is manufactured under a strict quality management system. Each batch is tested to ensure it meets high-purity standards suitable for sensitive molecular biology applications. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles is provided with every shipment to guarantee traceability and batch-to-batch consistency.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry, and well-ventilated area at room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure containers are sealed immediately after use to prevent absorption of atmospheric moisture, which can affect performance.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms
Assay (HPLC)	≥ 99.0%
pH (5% Solution)	4.5 - 7.0
Solubility (in Water)	Clear, colorless solution
Loss on Drying	≤ 0.5%
UV Absorbance (260 nm)	Passes test
RNase/DNase Activity	Not detected

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.