Description

2'-O-Methyl-5-Methyluridine 5'-Triphosphate Triethylammonium Salt is a chemically modified nucleoside triphosphate analog, essential for advanced molecular biology and therapeutic research. Its core value lies in providing enhanced stability and resistance to nuclease degradation, which is critical for applications requiring precise and durable nucleic acid manipulation. This high-purity biochemical is primarily needed by researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic sectors, particularly for developing oligonucleotide-based therapeutics, antisense technologies, and next-generation sequencing platforms.

Application

• Oligonucleotide Synthesis: A key building block for the solid-phase synthesis of chemically modified antisense oligonucleotides (ASOs) and siRNA, enhancing their metabolic stability and binding affinity.
• Therapeutic Development: Used in the research and production of novel nucleic acid therapeutics targeting genetic diseases, cancers, and viral infections.
• Next-Generation Sequencing (NGS): Employed as a substrate for engineered polymerases in sequencing-by-synthesis technologies to study epigenetic modifications.
• PCR and qPCR: Serves as a modified nucleotide triphosphate (dNTP) in specialized PCR applications to introduce specific properties into amplified DNA.
• Enzymatic Labeling: Utilized in enzymatic incorporation of labels (e.g., fluorescent or biotin tags) into nucleic acid probes for diagnostic assays.
• Structural Biology Studies: Applied in X-ray crystallography and NMR to investigate RNA structure, function, and protein-RNA interactions.

Basic Information

Product Name	2'-O-Methyl-5-Methyluridine 5'-Triphosphate Triethylammonium Salt
CAS No.	444789-41-7
Molecular Formula	C₁₈H₃₃N₄O₁₆P₃ (Free acid) • x(C₆H₁₅N)
Molecular Weight	Contact for details
Synonyms	2'-O-Me-5-Me-UTP, Triethylammonium salt; 5-Methyl-2'-O-methyluridine-5'-triphosphate; 2'-O-Methyl-5-methyl-UTP; m5Um-5'-triphosphate; NTP analog, 2'-O-methylated; 5-Methyl-2'-O-methyluridine triphosphate; Modified nucleoside triphosphate; Antisense oligonucleotide monomer
EINECS	Contact for details

Quality Control

Our 2'-O-Methyl-5-Methyluridine 5'-Triphosphate Triethylammonium Salt is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via NMR and MS, to ensure it meets the high standards required for pharmaceutical and life science research. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95%
Assay (UV)	≥ 90%
Water Content (KF)	≤ 5%
Triethylammonium Salt Content	Contact for details
Solubility	Soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.