Description

6-Fam-Dc-Puromycin is a fluorescently labeled puromycin derivative, a critical tool for studying protein synthesis and ribosome function. This compound enables the visualization and quantification of nascent polypeptides in live cells, providing a direct readout of translational activity. It is an essential reagent for researchers in molecular biology, biochemistry, and drug discovery who require high-purity, reliable probes for puromycin-based assays such as SUnSET and FUNCAT.

Application

• Visualization of Protein Synthesis: Direct labeling of newly synthesized proteins in live-cell imaging and fluorescence microscopy.
• Puromycin-Based Assays: Key component in Surface Sensing of Translation (SUnSET) and Fluorescent Non-Canonical Amino Acid Tagging (FUNCAT) methodologies.
• Ribosome Profiling: Used to capture and analyze actively translating ribosomes for high-throughput sequencing studies.
• Antibiotic Mechanism Studies: Research tool for investigating the mode of action of puromycin and related translation inhibitors.
• High-Content Screening (HCS): Fluorescent probe for automated, cell-based screening assays targeting protein translation pathways.
• Neuroscience Research: Mapping local protein synthesis in neuronal dendrites and synapses.

Basic Information

Product Name	6-Fam-Dc-Puromycin
CAS No.	436083-76-0
Molecular Formula	C₃₈H₄₀N₇O₁₄
Molecular Weight	818.77 g/mol
Synonyms	6-Carboxyfluorescein-DC-Puromycin; FAM-DC-Puromycin; 6-FAM-DC-Puro; 5(6)-Carboxyfluorescein-DC-Puromycin; Fluorescein-Labeled Puromycin; Puromycin-5(6)-carboxyfluorescein conjugate; N-((6-FAM)-aminocaproyl)puromycin
EINECS	Contact for details

Quality Control

Every batch of 6-Fam-Dc-Puromycin is manufactured and tested to meet stringent research-grade specifications. Our quality assurance includes comprehensive analytical characterization by HPLC, MS, and NMR to ensure identity, purity, and performance in biological assays. A detailed Certificate of Analysis (COA) documenting purity, fluorescence properties, and absence of critical impurities is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	Orange to red solid
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Absorption Maximum (λmax)	~492 nm
Emission Maximum (λem)	~518 nm
Solubility	Soluble in DMSO, DMF
Moisture Content (KF)	≤ 2.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.