Description

N2-Isobutyryl-5'-o-(4,4-Dimethoxytrityl)-2'-Deoxyguanosine-3'-Succinate, Tea Salt is a high-purity, protected nucleoside phosphoramidite building block, a critical intermediate in the automated synthesis of oligonucleotides. Its primary value lies in enabling the precise and efficient incorporation of deoxyguanosine into custom DNA sequences, ensuring high coupling efficiency and yield. This compound is essential for research institutions, diagnostic developers, and pharmaceutical companies engaged in therapeutic oligonucleotide development, genetic research, and molecular diagnostics. Consistent quality and reliable supply are paramount for downstream applications in drug discovery and advanced genomic technologies.

Application

• Oligonucleotide Synthesis: As a key phosphoramidite monomer for the solid-phase synthesis of DNA strands in automated synthesizers.
• Therapeutic API Manufacturing: Production of active pharmaceutical ingredients (APIs) for antisense oligonucleotides, siRNA, and other nucleic acid-based therapeutics.
• Research & Development: Synthesis of custom primers, probes, and gene fragments for PCR, sequencing, and other molecular biology techniques.
• Diagnostic Probe Production: Manufacture of labeled and unlabeled DNA probes used in diagnostic assays and genomic testing.
• CRISPR/Cas9 Component Synthesis: Building block for the production of guide RNAs (gRNAs) and other synthetic nucleic acid components.
• Antisense Technology: Core material for developing antisense oligonucleotides targeting specific mRNA sequences.

Basic Information

Product Name	N2-Isobutyryl-5'-o-(4,4-Dimethoxytrityl)-2'-Deoxyguanosine-3'-Succinate, Tea Salt
CAS No.	402944-21-2
Molecular Formula	C46H48N5O11 • C7H10N2O2
Molecular Weight	~ 1005.1 g/mol (Free acid)
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-N2-isobutyryl-2'-deoxyguanosine-3'-succinyl TEA salt; dG-Ibu DMT Succinate TEA Salt; N-Isobutyryl-5'-O-DMT-2'-deoxyguanosine-3'-(2-cyanoethyl N,N-diisopropylphosphoramidite) precursor; Protected Deoxyguanosine Phosphoramidite Intermediate; G(Ibu) DMT Suc TEA Salt; 2'-Deoxy-N2-isobutyrylguanosine, 5'-O-[bis(4-methoxyphenyl)phenylmethyl]-, 3'-(hydrogen succinate), triethylamine salt; Oligonucleotide Synthesis Building Block for dG
EINECS	Contact for details

Quality Control

Our production of this critical building block adheres to stringent quality protocols to ensure batch-to-batch consistency essential for automated synthesis. Quality is verified through comprehensive analytical methods including HPLC, NMR, and mass spectrometry. A Certificate of Analysis (COA) detailing purity, identity, and moisture content is provided with each shipment. We support compliance requirements for GMP-grade manufacturing of therapeutic oligonucleotides.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under anhydrous conditions. This material is hygroscopic (moisture-sensitive) and should be handled in a dry, inert atmosphere or with appropriate desiccant to maintain stability and reactivity. Allow the sealed container to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Moisture Content (KF)	≤ 1.0%
Solubility	Soluble in acetonitrile, dichloromethane

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.