Description

3',5'-Bis-o-(T-Butyldimethylsilyl)Thymidine is a key protected nucleoside derivative, specifically designed for advanced oligonucleotide synthesis. This compound is essential for researchers and manufacturers requiring high-purity intermediates to ensure the fidelity and yield of complex nucleic acid sequences. It is primarily utilized in the pharmaceutical, biotechnology, and diagnostic sectors for the development of antisense oligonucleotides, primers, and probes.

Application

• Oligonucleotide Synthesis: Serves as a critical phosphoramidite precursor in solid-phase synthesis for DNA and RNA sequences.
• Antisense Drug Development: Used in the research and production of therapeutic oligonucleotides targeting genetic diseases.
• Diagnostic Probe Manufacturing: A building block for labeled probes used in PCR, qPCR, and microarray technologies.
• Academic & Industrial Research: Enables chemical biology studies, including nucleotide modification and structure-activity relationship (SAR) investigations.
• Bioconjugation: Facilitates the site-specific modification of nucleic acids for targeted delivery or imaging applications.

Basic Information

Product Name	3',5'-Bis-o-(T-Butyldimethylsilyl)Thymidine
CAS No.	40733-26-4
Molecular Formula	C22H42N2O6Si2
Molecular Weight	486.76 g/mol
Synonyms	3',5'-Di-O-(tert-Butyldimethylsilyl)thymidine; 3',5'-Bis-O-(tert-butyldimethylsilyl)thymidine; 1-[(2R,4S,5R)-4-[[tert-Butyl(dimethyl)silyl]oxy]-5-[[tert-butyl(dimethyl)silyl]oxymethyl]oxolan-2-yl]-5-methyl-pyrimidine-2,4-dione; TBDMS-Thymidine; Thymidine 3',5'-bis(TBDMS) ether; 3',5'-Bis-O-TBDMS-thymidine; 3',5'-Di-O-TBDMS-thymidine
EINECS	Contact for details

Quality Control

Our 3',5'-Bis-o-(T-Butyldimethylsilyl)Thymidine is manufactured under strict quality systems to ensure batch-to-batch consistency for sensitive synthetic applications. Each lot undergoes comprehensive analytical testing, including HPLC, NMR, and mass spectrometry, to verify identity, purity, and absence of critical impurities. A Certificate of Analysis (COA) detailing all specifications is provided with every shipment to support your quality assurance and regulatory documentation.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and purity. For long-term storage, consider storage under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.