Description

5'-O-(Dimethoxytrityl)-N-(Dimethylamino)Methylidene-2'-Deoxypseudoisocytidine is a sophisticated, protected nucleoside derivative of significant importance in oligonucleotide synthesis. This compound serves as a critical building block for the solid-phase assembly of modified DNA and RNA strands, enabling advanced research and therapeutic development. It is primarily utilized by pharmaceutical R&D teams, biotechnology companies, and academic laboratories focused on antisense technology, siRNA, and aptamer development.

Application

• Oligonucleotide Synthesis: A key phosphoramidite precursor for the automated solid-phase synthesis of DNA and RNA sequences containing pseudoisocytidine analogs.
• Antisense & RNAi Therapeutics: Used in the development of chemically modified oligonucleotides for gene silencing applications, enhancing stability and binding affinity.
• Aptamer Development: Enables the creation of high-affinity nucleic acid ligands (aptamers) with expanded chemical diversity for diagnostic and therapeutic targeting.
• Structural Biology Studies: Incorporated into oligonucleotides to study nucleic acid structure, stability, and protein-nucleic acid interactions.
• Diagnostic Probe Design: Facilitates the production of specialized probes for molecular diagnostics, including FISH and PCR-based assays.
• Basic Research: Serves as a vital reagent for academic research exploring the biochemistry and pharmacology of modified nucleosides.

Basic Information

Product Name	5'-O-(Dimethoxytrityl)-N-(Dimethylamino)Methylidene-2'-Deoxypseudoisocytidine
CAS No.	307314-30-3
Molecular Formula	C34H38N4O6
Molecular Weight	598.69 g/mol
Synonyms	5'-O-DMT-N-(Dimethylamino)methylene-2'-deoxypseudoisocytidine; DMT-dPseudoC(DM) Amidite precursor; N4-(Dimethylaminomethylene)-5'-O-(4,4'-dimethoxytrityl)-2'-deoxypseudoisocytidine; 2'-Deoxy-N4-[(dimethylamino)methylene]pseudoisocytidine 5'-(4,4'-dimethoxytrityl) ether; Protected 2'-Deoxypseudoisocytidine; DMT-dψC(DM)
EINECS	Contact for details

Quality Control

Our production of 5'-O-(Dimethoxytrityl)-N-(Dimethylamino)Methylidene-2'-Deoxypseudoisocytidine adheres to stringent quality protocols suitable for research and pharmaceutical intermediate applications. Each batch is characterized and purified to ensure high chemical purity and consistency. Certificates of Analysis (COA) detailing identity, purity (HPLC), and impurity profiles are provided to support your quality assurance requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at -20°C or below for long-term stability. This material is hygroscopic (moisture-sensitive); allow the sealed container to equilibrate to room temperature before opening to prevent condensation. Store away from incompatible materials.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in acetonitrile, DMF, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.