Description

Tris Edta Buffer, Dnase, Rnase, Protease Free Ready To Use, Ph 8.0, For Molecular Biology is a high-purity, pre-formulated buffer solution designed for critical molecular biology applications. Its core value lies in being free from common enzymatic contaminants, ensuring the integrity of sensitive nucleic acid samples during cell lysis, washing, and storage procedures. This product is essential for researchers and technicians in biotechnology, pharmaceutical development, and academic laboratories who require reliable, ready-to-use reagents to minimize experimental variables and maximize reproducibility.

Application

• Nucleic Acid Extraction & Purification: Primary buffer for cell lysis and washing steps in plasmid DNA, genomic DNA, and RNA isolation kits.
• Molecular Cloning: Used in the preparation and resuspension of competent cells and for storing DNA ligation mixtures.
• Gel Electrophoresis: Serves as a running buffer or component in agarose and polyacrylamide gel electrophoresis (PAGE) of nucleic acids.
• Southern & Northern Blotting: Employed in denaturation, neutralization, and transfer buffer systems for membrane-based hybridization techniques.
• Restriction Enzyme Digestion: Provides a compatible buffering environment for many restriction endonucleases, often used in dilution or storage.
• Cell Culture & Microbiology: Used for washing bacterial pellets post-centrifugation and in various cell biology protocols requiring a Tris-based, metal-chelating buffer.
• Long-term DNA Storage: An ideal medium for the stable, long-term storage of purified DNA samples at 4°C or -20°C.
• Next-Generation Sequencing (NGS) Library Prep: Utilized in sample preparation workflows where nuclease-free conditions are paramount.

Basic Information

Product Name	Tris Edta Buffer, Dnase, Rnase, Protease Free Ready To Use, Ph 8.0, For Molecular Biology
CAS No.	38641-82-6
Molecular Formula	C10H19N3O8 • xH2O (Tris-EDTA complex)
Molecular Weight	389.28 g/mol (Anhydrous Tris-EDTA)
Synonyms	TE Buffer; Tris-EDTA Buffer Solution; Tris EDTA Buffer pH 8.0; Molecular Biology Grade TE Buffer; Nuclease-Free TE Buffer; DNase/RNase/Protease-Free Buffer; 1X TE Buffer; 10X TE Buffer; Tris(hydroxymethyl)aminomethane EDTA Buffer; DNA Storage Buffer
EINECS	Contact for details

Quality Control

Our products undergo rigorous quality testing to ensure compliance with industry standards. Each batch of this ready-to-use Tris-EDTA buffer is manufactured under controlled conditions and tested to confirm it meets stringent specifications for molecular biology applications. Key tests include verification of pH, absence of DNase, RNase, and protease activity, endotoxin levels, and bioburden. Certificates of Analysis (COA) are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at room temperature (15-25°C) or refrigerated (2-8°C) as per the specific product label. The ready-to-use solution is stable under these conditions for the duration of the shelf life.

Specification

Item	Specification
Appearance	Clear, colorless solution
pH (at 25°C)	8.0 ± 0.2
Tris Concentration	10 mM ± 10%
EDTA Concentration	1 mM ± 10%
DNase Activity	Not Detected
RNase Activity	Not Detected
Protease Activity	Not Detected
Endotoxin	< 0.1 EU/mL
Bioburden	< 10 CFU/mL
Filtered	0.22 µm sterile filtration

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.