Description

1,5-I-AEDANS is a specialized, heterobifunctional fluorescent labeling reagent primarily used for the site-specific modification of proteins and peptides. Its core value lies in its dual functionality, combining a reactive iodoacetamide group for thiol conjugation with a dansyl fluorophore for sensitive detection. This compound is essential for researchers and manufacturers in the fields of bioconjugation, proteomics, and fluorescence-based assay development, enabling advanced studies of protein structure, dynamics, and interactions.

Application

• Site-Specific Protein Labeling: Covalent attachment to cysteine residues for fluorescence tagging in structural biology.
• Fluorescence Resonance Energy Transfer (FRET) Studies: Serving as a donor or acceptor fluorophore in distance-measuring experiments.
• Protease Activity Assays: Development of fluorogenic substrates to monitor enzymatic cleavage.
• Bioconjugate Synthesis: Creating fluorescent antibody-drug conjugates (ADCs) or other diagnostic probes.
• Membrane Protein Studies: Labeling of thiol groups in membrane-bound proteins for fluorescence microscopy.
• Kinetic Binding Analysis: Monitoring real-time binding events in solution-phase assays.

Basic Information

Product Name	1,5-I-AEDANS
CAS No.	36930-63-9
Molecular Formula	C20H23IN2O4S
Molecular Weight	514.38 g/mol
Synonyms	N-(Iodoacetyl)-N'-(5-sulfo-1-naphthyl)ethylenediamine; 5-((((2-Iodoacetyl)amino)ethyl)amino)naphthalene-1-sulfonic acid; 1,5-IAEDANS; IAEDANS; AEDANS iodoacetamide; I-AEDANS; Fluorescent thiol-reactive probe
EINECS	Contact for details

Quality Control

Our 1,5-I-AEDANS is manufactured under strict quality protocols to ensure high purity and batch-to-batch consistency, suitable for sensitive research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance characteristics. We adhere to ISO 9001 quality management standards, and our processes are designed to meet the rigorous demands of analytical and life science research.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This product is light-sensitive (store away from light) and should be allowed to warm to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 2.0%
Fluorescence Properties	Ex/Em ~336/490 nm (verify in buffer)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.