Description

Uracil, [5,6-3H] is a tritium-labeled derivative of the essential pyrimidine nucleobase uracil, with the radiolabel specifically incorporated at the 5 and 6 positions. This compound is a critical tool for researchers requiring high-sensitivity detection and quantification in metabolic and biochemical studies. It is primarily utilized in life science research, pharmaceutical development, and diagnostic applications where precise tracing of uracil incorporation and metabolism is necessary.

Application

• Biochemical Research: As a radiolabeled tracer in studies of pyrimidine metabolism, nucleotide salvage pathways, and RNA synthesis.
• Drug Discovery & Development: For investigating the mechanism of action and cellular uptake of anticancer and antiviral agents that target nucleotide metabolism (e.g., 5-fluorouracil).
• Metabolic Pathway Analysis: Enabling autoradiography and liquid scintillation counting to visualize and quantify uracil utilization in cell cultures and tissue samples.
• Enzyme Kinetics Studies: Serving as a substrate for assays involving enzymes like uridine phosphorylase or dihydropyrimidine dehydrogenase (DPD).
• Diagnostic Probe Development: Potential use in the development of diagnostic assays for conditions related to aberrant pyrimidine metabolism.

Basic Information

Product Name	Uracil, [5,6-3H]
CAS No.	35978-19-9
Molecular Formula	C4H43H2N2O2
Molecular Weight	~114.1 g/mol (exact value depends on specific activity)
Synonyms	2,4(1H,3H)-Pyrimidinedione, 5,6-tritio-; [5,6-3H]Uracil; Tritiated Uracil; 3H-Uracil; Uracil-5,6-3H; Uracil (tritiated); Radiolabeled Uracil; Pyrimidine-2,4-dione-5,6-3H
EINECS	Contact for details

Quality Control

Every batch of Uracil, [5,6-3H] is manufactured and analyzed under controlled conditions to ensure high radiochemical purity and specific activity. Quality is verified by techniques including HPLC with radiometric detection and TLC. A comprehensive Certificate of Analysis (COA) is provided with each shipment, detailing specific activity, radiochemical purity, chemical purity, and solvent information. Our quality system supports research compliance standards.

Storage

Preserve in a tightly closed container, protected from light. Store at or below -20°C in the original solvent as supplied. For long-term storage, keep at -70°C to minimize radiolytic decomposition. Always handle in accordance with local radiation safety regulations and guidelines for radioactive materials.

Specification

Item	Specification
Appearance	Clear, colorless to slightly yellow solution
Specific Activity	10-40 Ci/mmol (Contact for exact value)
Radiochemical Purity	≥ 97% (by HPLC)
Chemical Purity	≥ 98%
Concentration	1 mCi/mL (Contact for other concentrations)
Solvent	Ethanol:Water (7:3) or as specified

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.