Description

5-Iodouridine 5'-Triphosphate Sodium Salt - 100Mm Solution is a chemically modified nucleotide triphosphate analog, supplied as a ready-to-use 100 mM aqueous solution. This product is essential for enzymatic nucleic acid labeling and sequencing applications, where it serves as a substrate for DNA and RNA polymerases. It is primarily used by researchers and manufacturers in the fields of molecular biology, diagnostics, and next-generation sequencing to introduce specific labels or probes into nucleic acid chains.

Application

• Next-Generation Sequencing (NGS): Used as a modified nucleotide for sequencing-by-synthesis platforms.
• DNA/RNA Labeling: Acts as a substrate for polymerases to incorporate iodine-labeled nucleotides for detection or crosslinking studies.
• Probe Synthesis: Essential for creating labeled nucleic acid probes used in fluorescence in situ hybridization (FISH) and microarray technologies.
• Enzymatic Assays: Serves as a critical reagent in polymerase activity assays and mechanistic studies of nucleic acid enzymes.
• Structural Biology: Used in X-ray crystallography and other structural studies where heavy atom incorporation is required for phasing.
• Diagnostic Kit Manufacturing: A key raw material in the production of kits for pathogen detection and genetic testing.

Basic Information

Product Name	5-Iodouridine 5'-Triphosphate Sodium Salt - 100Mm Solution
CAS No.	34198-43-1
Molecular Formula	C9H11IN2Na2O14P3 (for the sodium salt)
Molecular Weight	Contact for details
Synonyms	5-Iodo-UTP sodium salt; 5-Iodouridine-5'-triphosphate trisodium salt; Iodo-UTP; 5-Iodo-2'-deoxyuridine-5'-triphosphate sodium salt (related compound); 5-Iodouridine triphosphate; ITP (context-dependent); 5-Iodo-Uridine-5'-triphosphoric acid sodium salt; UTP-5-I
EINECS	Contact for details

Quality Control

Our 5-Iodouridine 5'-Triphosphate Sodium Salt solution is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity verification, to ensure it meets the high standards required for sensitive life science applications. Certificates of Analysis (COA) with detailed specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store the 100 mM solution at -20°C or below. Avoid repeated freeze-thaw cycles. The product is light-sensitive (store away from light).

Specification

Item	Specification
Appearance	Clear, colorless to slightly yellow solution
Concentration	100 mM ± 5% (in aqueous solution)
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 95%
pH	6.5 - 8.5
Endotoxin	< 1.0 EU/mL
Filter Sterilization	0.2 µm filtered

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.