Description

Guanosine 3'-(Trihydrogen Diphosphate), 5'-(Trihydrogen Diphosphate) is a high-purity nucleotide derivative, also known as guanosine 3',5'-bis(diphosphate) or ppGpp. This compound is a critical signaling molecule, playing a fundamental role in the bacterial stringent response to nutrient stress. It is essential for researchers in microbiology, molecular biology, and pharmaceutical development who require reliable, high-quality biochemical reagents for studying bacterial physiology, antibiotic resistance mechanisms, and metabolic regulation.

Application

• Biochemical Research: A key reagent for studying the bacterial stringent response and (p)ppGpp signaling pathways.
• Antibiotic Development: Used in target-based screening and mechanism-of-action studies for novel antibacterial agents.
• Enzymology Studies: Serves as a substrate or effector for enzymes like RelA and SpoT synthetases/hydrolases.
• Microbial Physiology: Investigates bacterial adaptation, persistence, biofilm formation, and virulence regulation.
• Diagnostic Assay Development: Potential use in developing biosensors or assays for bacterial stress response detection.
• Synthetic Biology: Used to engineer and control bacterial metabolic networks and growth behaviors.

Basic Information

Product Name	Guanosine 3'-(Trihydrogen Diphosphate), 5'-(Trihydrogen Diphosphate)
CAS No.	32452-17-8
Molecular Formula	C10H17N5O17P4
Molecular Weight	603.17 g/mol
Synonyms	Guanosine 3',5'-Bis(diphosphate); ppGpp; Guanosine Pentaphosphate; Magic Spot I; MS I; Guanosine 3'-diphosphate 5'-diphosphate; Alarmone ppGpp; Guanosine 5'-diphosphate 3'-diphosphate
EINECS	Contact for details

Quality Control

Our Guanosine 3',5'-bis(diphosphate) is manufactured under strict quality systems to ensure batch-to-batch consistency and reliability for sensitive research applications. Each lot undergoes comprehensive analytical characterization, including HPLC for purity and identity confirmation via mass spectrometry. Certificates of Analysis (COA) detailing all specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Water Content (Karl Fischer)	≤ 5.0 %
Biological Activity	Tested in stringent response assay (Contact for details)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.