Description

Guanosine 5'-(Beta,γ-Methylene)Triphosphate is a non-hydrolyzable analog of GTP, where a methylene group replaces the oxygen between the beta and gamma phosphates. This compound is critical for studying GTP-binding proteins (G-proteins) and GTPases, as it locks them in an active conformation, enabling detailed mechanistic and structural analysis. It is an essential biochemical tool for researchers in molecular biology, enzymology, and signal transduction, providing insights into cellular regulation and disease mechanisms.

Application

• G-protein and GTPase Research: Used as a non-hydrolyzable activator to study the structure and function of regulatory proteins like Ras, Rho, and heterotrimeric G-proteins.
• Enzyme Kinetics & Mechanism Studies: Serves as a substrate analog to investigate the catalytic cycle, binding affinity, and transition states of nucleotide-dependent enzymes.
• Structural Biology (X-ray Crystallography & Cryo-EM): Employed to trap and stabilize GTP-binding proteins in their active state for high-resolution structural determination.
• Signal Transduction Pathway Analysis: Essential for in vitro assays to dissect activation and regulation mechanisms in key cellular signaling pathways.
• Drug Discovery & Screening: Used in high-throughput screening assays to identify potential inhibitors or modulators of oncogenic GTPases (e.g., KRAS).
• Biochemical Reagent Manufacturing: A key component in the formulation of specialized assay kits for research and diagnostic development.

Basic Information

Product Name	Guanosine 5'-(Beta,γ-Methylene)Triphosphate
CAS No.	30100-88-0
Molecular Formula	C11H17N5O12P3
Molecular Weight	520.19 g/mol
Synonyms	GMP-PCP; Guanosine 5'-[β,γ-methylene]triphosphate; GMPPCP; β,γ-Methylene-guanosine-5'-triphosphate; Guanosine-5'-(β,γ-methylenetriphosphate); Guanylyl methylenediphosphonate; Gpp(CH2)p; Non-hydrolyzable GTP analog
EINECS	Contact for details

Quality Control

Our Guanosine 5'-(Beta,γ-Methylene)Triphosphate is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity for sensitive research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and NMR for structural confirmation. Certificates of Analysis (COA) with detailed specifications are provided and available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0 %
Solubility	Soluble in water or buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.