Description

Thymine-Methyl-3H Mol. Wt. 126.1 CAS NO 3590-50-9 is a labeled derivative of thymine, a fundamental pyrimidine nucleobase, where a tritium (³H) isotope is incorporated at the methyl group. This compound is critical for advanced research and development, providing a radioactive tracer for precise metabolic and biochemical pathway studies. It is essential for pharmaceutical R&D, academic research, and diagnostic development in life sciences, enabling detailed investigation into DNA synthesis, cell proliferation, and nucleoside analog metabolism.

Application

• Biochemical Tracer Studies: Used as a radiolabeled precursor in metabolic pathway analysis and pharmacokinetic research.
• Nucleic Acid Research: Essential for investigating DNA synthesis, repair mechanisms, and the function of thymidine kinase.
• Antiviral & Anticancer Drug Development: Serves as a key tool in the development and testing of nucleoside analog therapeutics.
• Autoradiography & Imaging: Employed in molecular biology techniques for visualizing and quantifying biological processes at the cellular level.
• Enzyme Assay Substrate: Used in assays to study enzyme activity, particularly those involved in nucleotide metabolism.
• Diagnostic Probe Development: Applied in the creation of sensitive probes for various diagnostic applications.

Basic Information

Product Name	Thymine-Methyl-3H Mol. Wt. 126.1 CAS NO 3590-50-9
CAS No.	3590-50-9
Molecular Formula	C₆H₆³H₂N₂O₂
Molecular Weight	126.1 g/mol
Synonyms	5-Methyl-2,4(1H,3H)-pyrimidinedione-3H (methyl-³H); [Methyl-³H]Thymine; Thymine (methyl-tritiated); 5-Methyluracil-³H (methyl); 2,4-Dihydroxy-5-methylpyrimidine-³H₃; 5-Methyl-³H₃-pyrimidine-2,4(1H,3H)-dione; NSC 21548-³H; Radioactive Thymine
EINECS	Contact for details

Quality Control

Every batch of Thymine-Methyl-3H is manufactured and tested under strict quality systems to ensure high specific activity, radiochemical purity, and chemical stability. Our quality protocols include rigorous analysis via HPLC and spectroscopic methods. A comprehensive Certificate of Analysis (COA), detailing specific activity, purity, and solvent composition, is provided with each shipment to support your regulatory and research documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C or as specified on the label. Due to its nature as a light-sensitive and easily oxidized (store under inert atmosphere) compound, it is recommended to store the material under an inert atmosphere when possible and minimize exposure to air and moisture during use.

Specification

Item	Specification
Appearance	White to off-white solid or solution
Identification (IR)	Conforms to structure
Specific Activity	Customizable (e.g., 10-100 Ci/mmol)
Radiochemical Purity (HPLC)	≥ 95%
Chemical Purity (HPLC)	≥ 98%
Solvent	Ethanol/Water or as specified

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.