Description

T-Boc-Aminooxy-Peg4-Nhs Ester is a heterobifunctional polyethylene glycol (PEG) crosslinker featuring an amine-reactive NHS ester and an aminooxy group protected by a tert-butyloxycarbonyl (T-Boc) group. This compound is essential for creating stable, site-specific conjugations between amine-containing molecules (like proteins or peptides) and carbonyl-containing targets under mild, controlled conditions. It is primarily utilized by researchers and process developers in bioconjugation, pharmaceutical development, and advanced materials science to engineer novel therapeutics, diagnostics, and biomaterials.

Application

• Bioconjugation and ADC Development: Site-specific coupling of antibodies, proteins, or peptides to drugs, toxins, or fluorophores for creating antibody-drug conjugates (ADCs) and other biopharmaceuticals.
• PEGylation Chemistry: Introduction of a hydrophilic PEG spacer to biomolecules to enhance solubility, prolong circulation half-life, and reduce immunogenicity.
• Surface Functionalization: Modification of nanoparticles, quantum dots, or biosensor surfaces with aminooxy groups for subsequent oxime ligation with aldehydes or ketones.
• Hydrogel and Biomaterial Synthesis: Crosslinking agent for creating hydrogels and polymer networks with tailored degradation profiles and bioactive properties.
• Chemical Biology Probes: Synthesis of activity-based probes or affinity labels for studying protein function and interactions in biological systems.
• Diagnostic Reagent Preparation: Conjugation of enzymes or other reporter molecules to antibodies or oligonucleotides for ELISA, lateral flow assays, and other diagnostic platforms.

Basic Information

Product Name	T-Boc-Aminooxy-Peg4-Nhs Ester
CAS No.	2401831-99-8
Molecular Formula	C26H46N2O12
Molecular Weight	578.66 g/mol
Synonyms	N-(t-Boc-aminooxy)-PEG4-NHS ester; 2,5-Dioxopyrrolidin-1-yl 3-[2-[2-[2-[2-[(2-methylpropan-2-yl)oxycarbonylamino]oxyethoxy]ethoxy]ethoxy]ethoxy]propanoate; Boc-NH-O-PEG4-NHS; tert-Butyl N-[(17-oxo-4,7,10,13-tetraoxa-16-azaheptadec-1-yl)oxy]carbamate; Aminooxy-PEG4-NHS ester, Boc-protected; T-Boc protected aminooxy-PEG4-NHS ester; Boc-Aminooxy-PEG4-NHS; Boc-N-amidooxy-PEG4-NHS Ester
EINECS	Contact for details

Quality Control

Our T-Boc-Aminooxy-Peg4-Nhs Ester is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing to ensure high purity and consistency, meeting the stringent requirements for research and pharmaceutical development. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles by HPLC, NMR, and MS is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This material is hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (HPLC/MS)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.