Description

Carboxy Fluorescein-Peg12-Nhs is a high-purity, amine-reactive fluorescent labeling reagent designed for advanced bioconjugation. This compound is critical for researchers and manufacturers who require precise, stable, and water-soluble fluorescent tags for biomolecules such as proteins, peptides, and antibodies. It is essential for applications in life sciences, diagnostics, and pharmaceutical development where reliable and sensitive detection is paramount.

Application

• Bioconjugation and Protein Labeling: Primary use for covalently attaching a bright, stable fluorescein derivative to primary amines (-NH2) on proteins, antibodies, and peptides for detection and analysis.
• Fluorescence Microscopy & Imaging: Creating labeled probes for cellular imaging, tracking intracellular processes, and studying protein localization and dynamics.
• Flow Cytometry & Diagnostics: Development of fluorescently-tagged antibodies and other detection reagents for diagnostic assays, cell sorting, and immunophenotyping.
• Biomolecular Assay Development: Used in FRET (Förster Resonance Energy Transfer) assays, ELISA, and other plate-based detection systems requiring a high signal-to-noise ratio.
• Drug Delivery & Biopharmaceutical Research: Labeling of drug carriers, nanoparticles, and therapeutic proteins to study pharmacokinetics, biodistribution, and target engagement.
• Material Science & Bioengineering: Functionalization of surfaces and biomaterials with fluorescent markers for visualization and quality control in engineered tissues or biosensors.

Basic Information

Product Name	Carboxy Fluorescein-Peg12-Nhs
CAS No.	2246595-66-2
Molecular Formula	C71H96N4O31
Molecular Weight	1501.55 g/mol
Synonyms	5(6)-Carboxyfluorescein-PEG12-NHS ester; 6-FAM-PEG12-NHS; Carboxyfluorescein-Polyethylene glycol12-N-Hydroxysuccinimide ester; 5(6)-FAM-PEG12-NHS; CF-PEG12-NHS; Fluorescein-PEG12-NHS; 5(6)-Carboxyfluorescein, 12-Unit PEG spacer, NHS ester; Succinimidyl ester of Carboxyfluorescein-PEG12
EINECS	Contact for details

Quality Control

Our Carboxy Fluorescein-Peg12-Nhs is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation (NMR, MS), to ensure it meets the high standards required for sensitive research and development applications. Certificates of Analysis (COA) detailing purity, absorbance, and functional performance are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to warm to room temperature in a desiccator before opening to minimize moisture absorption. For long-term stability, store under inert atmosphere.

Specification

Item	Specification
Appearance	Orange to red powder or solid
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Absorption Maximum (λmax)	~492 nm
Emission Maximum	~518 nm
Extinction Coefficient (ε)	~80,000 cm-1M-1
Solubility	Soluble in DMSO, DMF, acetonitrile; sparingly soluble in water
Water Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.