Description

Methyltetrazine-Peg12-Dbco CAS NO 2183440-28-8 is a high-purity, heterobifunctional crosslinking reagent designed for advanced bioconjugation and click chemistry applications. Its unique structure combines a methyltetrazine group for rapid, bioorthogonal inverse electron-demand Diels-Alder (IEDDA) reactions with a DBCO moiety for strain-promoted azide-alkyne cycloaddition (SPAAC), interconnected by a long, hydrophilic PEG12 spacer. This product is essential for researchers and developers in the pharmaceutical and diagnostics sectors who require precise, efficient, and modular strategies for labeling biomolecules, constructing antibody-drug conjugates (ADCs), and developing complex therapeutic nanomaterials.

Application

• Antibody-Drug Conjugate (ADC) Construction: Enables the site-specific, multi-step conjugation of cytotoxic payloads to monoclonal antibodies via orthogonal click chemistry handles.
• Biomolecule Labeling & Detection: Used for fluorescent or affinity tagging of proteins, peptides, oligonucleotides, and other biomolecules in assay development and cellular imaging.
• Surface Functionalization: Facilitates the modification of nanoparticles, microspheres, biosensors, and microarray slides with bioactive ligands for targeted delivery or diagnostic capture.
• Polymer & Material Science: Serves as a key building block for synthesizing smart, functionalized hydrogels, dendrimers, and other polymeric materials with tailored biological activity.
• Pre-Targeting Strategies in Radioimmunotherapy: Critical component in multi-step pre-targeting approaches, where the tetrazine group allows for rapid in vivo capture of radiolabeled probes.
• Chemical Biology Probes: Employed in the development of activity-based probes and chemoproteomic tools to study enzyme function and protein interactions.

Basic Information

Product Name	Methyltetrazine-Peg12-Dbco
CAS No.	2183440-28-8
Molecular Formula	C73H116N6O27
Molecular Weight	1533.7 g/mol (approx.)
Synonyms	DBCO-PEG12-Methyltetrazine; Methyltetrazine-PEG12-DBCO; DBCO-PEG12-Methyltetrazine; (1R,8S,9s)-Bicyclo[6.1.0]non-4-yn-9-ylmethyl (1-((2-(2-(2-(2-(2-(2-(2-(2-(2-(2-(2-(2-(6-Methyl-1,2,4,5-tetrazin-3-yl)ethoxy)ethoxy)ethoxy)ethoxy)ethoxy)ethoxy)ethoxy)ethoxy)ethoxy)ethoxy)ethoxy)ethoxy)ethyl)carbamate; Methyl-Tetrazine-PEG12-DBCO; DBCO-PEG12-Tetrazine; Heterobifunctional PEG linker DBCO-PEG12-Methyltetrazine
EINECS	Contact for details

Quality Control

Our Methyltetrazine-Peg12-Dbco is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical characterization, including HPLC for purity assessment, NMR (1H, 13C) for structural confirmation, and mass spectrometry (MS) for identity verification. We ensure the product meets the high standards required for sensitive research and development applications. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting all relevant specifications and test results.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C or below under an inert atmosphere (e.g., argon or nitrogen), as the compound is easily oxidized and light-sensitive. Allow the vial to warm to room temperature in a desiccator before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Yellow to orange viscous oil or solid
Identification (NMR)	Complies with structure
Identification (MS)	Complies (M+Na+ or M+H+ peak observed)
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile; partially soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.