Description

n-Peg3-N'-(Propargyl-Peg4)-Cy5 CAS NO 2107273-06-1 is a heterobifunctional, fluorescent labeling reagent designed for advanced bioconjugation and bioimaging applications. This compound is engineered to provide a critical link between biomolecules and detection systems, combining a near-infrared cyanine dye (Cy5) with orthogonal click chemistry handles. It is essential for researchers and developers in the fields of biopharmaceuticals, diagnostic assay development, and advanced materials science who require precise, stable, and high-performance fluorescent probes.

Application

• Antibody-Drug Conjugate (ADC) Development: Site-specific conjugation of cytotoxic payloads or imaging agents to monoclonal antibodies via the propargyl-PEG4 alkyne group.
• Fluorescent Labeling of Biomolecules: Labeling of proteins, peptides, oligonucleotides, and other biomolecules with the bright, near-infrared Cy5 fluorophore for detection and tracking.
• Bioimaging Probe Synthesis: Creation of targeted imaging agents for in vitro and in vivo fluorescence microscopy, flow cytometry, and small animal imaging.
• Click Chemistry Reagent: Utilized in copper-catalyzed azide-alkyne cycloaddition (CuAAC) or strain-promoted azide-alkyne cycloaddition (SPAAC) reactions for modular assembly of complex molecular architectures.
• Polymer and Materials Functionalization: Introduction of fluorescent properties and bio-reactive groups into hydrogels, nanoparticles, and other polymeric materials.
• Diagnostic Kit Manufacturing: Key component in the production of lateral flow assays, ELISA kits, and other diagnostic tools requiring stable fluorescent signals.

Basic Information

Product Name	n-Peg3-N'-(Propargyl-Peg4)-Cy5
CAS No.	2107273-06-1
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Propargyl-PEG4-Cy5-PEG3-NHS ester; Cy5-PEG3-NHS-PEG4-Propargyl; Cy5-PEG3-PEG4-Alkyne NHS ester; Alkyne-PEG4-Cy5-PEG3-NHS; NHS-PEG3-Cy5-PEG4-Propargyl; Heterobifunctional Cy5-PEG linker with NHS and alkyne; Cy5-PEG3-NHS ester-PEG4-Propargyl; Propargyl-PEG4-Cy5-PEG3-NHS Ester
EINECS	Contact for details

Quality Control

Our n-Peg3-N'-(Propargyl-Peg4)-Cy5 is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, mass spectrometry for identity confirmation, and functional group analysis to ensure optimal performance in conjugation reactions. Certificates of Analysis (COA) detailing purity, identity, and other critical parameters are available upon request to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and light-sensitive. For long-term stability, store in a freezer and allow the vial to equilibrate to room temperature before opening to minimize condensation and moisture uptake.

Specification

Item	Specification
Appearance	Dark blue to purple solid or film
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Functional Group Integrity (NHS)	≥ 90% active ester
Functional Group Integrity (Alkyne)	≥ 90%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.