Description

H-Ap53 Primer (2 μ CAS NO 299935-15-2 is a specialized oligonucleotide primer designed for molecular biology and genetic research applications. This high-purity synthetic DNA sequence is critical for the specific amplification and detection of target gene sequences in polymerase chain reaction (PCR) and related techniques. It is an essential reagent for researchers and diagnostic developers in pharmaceutical R&D, academic institutions, and biotechnology companies focusing on genetic analysis, mutation detection, and assay development.

Application

• Polymerase Chain Reaction (PCR): Used as a forward or reverse primer for the specific amplification of target DNA sequences in conventional, quantitative (qPCR), and reverse transcription (RT-PCR) protocols.
• Genetic Research & Mutation Analysis: Essential for studies involving specific gene loci, including genotyping, allele discrimination, and the detection of single nucleotide polymorphisms (SNPs).
• Diagnostic Assay Development: A key component in the design and manufacturing of in-vitro diagnostic (IVD) kits for clinical testing and disease monitoring.
• Next-Generation Sequencing (NGS) Library Preparation: Can be utilized as a target-specific primer in amplicon-based NGS workflows for focused genomic studies.
• Academic & Pharmaceutical R&D: Supports fundamental biological research, target validation, and drug discovery processes in laboratory settings.

Basic Information

Item	Detail
Product Name	H-Ap53 Primer (2 μ CAS NO 299935-15-2
CAS No.	299935-15-2
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Ap53 Primer; H-Ap53 Oligonucleotide; DNA Primer Ap53; Oligo d(Ap53); Synthetic DNA Primer; PCR Primer for Ap53; 299935-15-2; Ap53 Forward/Reverse Primer
EINECS	Contact for details

Quality Control

Our oligonucleotide primers are manufactured under strict quality-controlled conditions to ensure batch-to-batch consistency and performance. Each lot undergoes rigorous analytical testing, including HPLC purification for purity and mass spectrometry (MS) verification for sequence accuracy and molecular weight confirmation. Certificates of Analysis (COA) detailing purity, concentration, and quality specifications are available upon request to support your research and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store lyophilized powder at -20°C. Once reconstituted in nuclease-free buffer or water, aliquot and store at -20°C to minimize freeze-thaw cycles. The product is sensitive to nucleases; use aseptic techniques.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Purity (HPLC)	≥ 95%
Identification (Mass Spec)	Conforms to theoretical mass
Sequence Verification	Conforms to specified sequence
Oligo Length	Specified base pairs
Concentration	As specified (e.g., 100 µM in TE buffer)
Endotoxin Level	< 0.1 EU/µmol (if applicable)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.