Description

5'-Dabcyl Cep CAS NO 288631-58-3 is a specialized, high-purity chemical intermediate primarily used as a key building block in the synthesis of advanced oligonucleotide probes and diagnostic reagents. Its value lies in its role as a critical quencher moiety in fluorescence resonance energy transfer (FRET) systems, enabling highly sensitive detection and quantification in molecular biology assays. This product is essential for manufacturers and research institutions in the fields of molecular diagnostics, next-generation sequencing (NGS), and pharmaceutical research, where precision and reliability are paramount.

Application

• Molecular Beacon Synthesis: As a dark quencher (Dabcyl) component in dual-labeled oligonucleotide probes for real-time PCR and other nucleic acid detection technologies.
• FRET-Based Assay Development: For constructing probes used in studying enzyme activity, protein-protein interactions, and intracellular signaling pathways.
• Diagnostic Kit Manufacturing: A critical raw material for producing commercial diagnostic tests for infectious diseases, genetic disorders, and oncology.
• Advanced Research Reagents: Used by academic and industrial R&D labs in genomics, proteomics, and high-throughput screening applications.
• Oligonucleotide Therapeutics: As a component in the research and development of antisense oligonucleotides and other therapeutic nucleic acids.
• Biosensor Fabrication: Integration into sensor platforms for the detection of specific DNA/RNA sequences with high specificity.

Basic Information

Product Name	5'-Dabcyl Cep
CAS No.	288631-58-3
Molecular Formula	C44H49N4O9P
Molecular Weight	808.86 g/mol
Synonyms	5'-Dabcyl-CE Phosphoramidite; 4-((4-(Dimethylamino)phenyl)azo)benzoic Acid, 2-Cyanoethyl N,N-Diisopropylphosphoramidite Derivative; Dabcyl Phosphoramidite; DABCYL CE Phosphoramidite; 5'-DABCYL; 4-Dimethylaminophenylazobenzoic Acid-CE Phosphoramidite; Dark Quencher Phosphoramidite
EINECS	Contact for details

Quality Control

Every batch of 5'-Dabcyl Cep is manufactured under strict quality management systems and undergoes comprehensive analytical testing to ensure it meets the exacting standards required for sensitive life science applications. We provide a detailed Certificate of Analysis (COA) with each shipment, confirming identity, purity, and performance-critical parameters. Our quality commitment supports compliance with ISO 13485 standards for medical device components and cGMP guidelines where applicable.

Storage

Preserve in a tightly closed container, protected from light. Store in a freezer at -20°C ± 5°C under anhydrous conditions. This product is hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation and moisture ingress. For long-term storage, keep under an inert atmosphere (argon or nitrogen).

Specification

Item	Specification
Appearance	Red to dark red solid or viscous oil
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in acetonitrile, dichloromethane, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.