Description

2'-o-(tert-Butyldimethylsilyl)-5'-O (P,P'-Dimethoxytrityl)Inosine 3'-[(2-Cyanoethyl)N,n-Diisopropylaminophosphoramidite] is a highly specialized phosphoramidite monomer used in the automated solid-phase synthesis of oligonucleotides. This compound is critical for the precise and efficient incorporation of inosine, a nucleoside analog with unique base-pairing properties, into synthetic DNA and RNA strands. It is an essential raw material for research institutions, diagnostic kit developers, and pharmaceutical companies engaged in antisense therapy, aptamer development, and molecular biology research.

Application

• Oligonucleotide Synthesis: Primary building block for the automated solid-phase synthesis of DNA/RNA strands containing inosine residues.
• Antisense & siRNA Research: Used in the production of research-grade antisense oligonucleotides and small interfering RNAs (siRNAs) where inosine is utilized for its degenerate base-pairing capability.
• Aptamer Development: Critical for creating nucleic acid aptamers with enhanced binding diversity and specificity for target proteins or molecules.
• PCR Primers & Probes: Manufacture of primers and probes containing inosine to reduce degeneracy in primer mixtures or to probe ambiguous genomic regions.
• Therapeutic Oligonucleotide Manufacturing: Serves as a key starting material in the GMP-compliant production of oligonucleotide-based therapeutics.
• Molecular Diagnostics: Used in synthesizing components for diagnostic assays and next-generation sequencing platforms.

Basic Information

Product Name	2'-o-(tert-Butyldimethylsilyl)-5'-O (P,P'-Dimethoxytrityl)Inosine 3'-[(2-Cyanoethyl)N,n-Diisopropylaminophosphoramidite]
CAS No.	261518-12-1
Molecular Formula	C48H64N7O8PSi
Molecular Weight	926.12 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-2'-O-(tert-butyldimethylsilyl)inosine 3'-(2-cyanoethyl N,N-diisopropylphosphoramidite); DMTr-iBu-Inosine Phosphoramidite; Inosine Phosphoramidite (2'-O-TBDMS protected); 2'-O-TBDMS-5'-O-DMTr-Inosine-3'-CE Phosphoramidite; N6-DMTr-2'-O-TBDMS-Inosine-3'-phosphoramidite; Inosine CE Phosphoramidite; dI Phosphoramidite (2'-O-TBDMS); 5'-DMTr-2'-TBDMS-Inosines-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidit
EINECS	Contact for details

Quality Control

This high-purity phosphoramidite is manufactured under strict quality control protocols to ensure batch-to-batch consistency and optimal coupling efficiency. Each lot is characterized by HPLC, 31P NMR, and 1H NMR to confirm identity, purity, and the absence of critical impurities. A comprehensive Certificate of Analysis (COA) detailing all specifications is provided with every shipment. Our production adheres to quality management systems suitable for demanding research and pre-clinical applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a dry, moisture-free environment. This product is hygroscopic (moisture-sensitive) and easily oxidized. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation. For long-term stability, store desiccated at -20°C.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (31P NMR)	Conforms to reference standard
Purity (HPLC)	≥ 97.0%
Water Content (KF)	≤ 1.0%
Coupling Efficiency Test	≥ 98.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.