Description

1-((3S,4R,5S)-5-((Bis(4-Methoxyphenyl)(Phenyl)Methoxy)Methyl)-4-Hydroxytetrahydrofuran-3-Yl)-5-Methylpyrimidine-2,4(1H,3H)-Dione is a sophisticated nucleoside derivative and a key protected intermediate in oligonucleotide synthesis. Its primary value lies in enabling the precise, automated construction of DNA and RNA sequences, which is fundamental to modern genetic research and therapeutic development. This high-purity compound is essential for pharmaceutical R&D teams, diagnostic developers, and academic laboratories working on antisense oligonucleotides, siRNA, aptamers, and other advanced nucleic acid-based technologies.

Application

• Solid-Phase Oligonucleotide Synthesis (SPOS): Serves as a critical phosphoramidite building block for the automated, stepwise assembly of DNA and RNA strands.
• Antisense Oligonucleotide (ASO) Development: Used in the research and production of therapeutic ASOs designed to modulate gene expression for treating genetic disorders, cancers, and viral infections.
• Small Interfering RNA (siRNA) Manufacturing: A key raw material in the synthesis of siRNA duplexes for RNA interference (RNAi) applications in both research and gene-silencing therapies.
• Diagnostic Probe Synthesis: Employed in creating labeled DNA/RNA probes for techniques like FISH (Fluorescence In Situ Hybridization), PCR, and next-generation sequencing.
• Aptamer and Nucleic Acid Library Construction: Essential for generating vast libraries of random oligonucleotides used in SELEX (Systematic Evolution of Ligands by Exponential Enrichment) processes to identify high-affinity aptamers.
• Research & Development of Novel Therapeutics: Supports exploratory research into new modalities including CRISPR guide RNAs, mRNA vaccines, and gene editing components.

Basic Information

Product Name	1-((3S,4R,5S)-5-((Bis(4-Methoxyphenyl)(Phenyl)Methoxy)Methyl)-4-Hydroxytetrahydrofuran-3-Yl)-5-Methylpyrimidine-2,4(1H,3H)-Dione
CAS No.	258524-54-8
Molecular Formula	C31H32N2O7
Molecular Weight	544.60 g/mol
Synonyms	5-Methyl-dT-CE Phosphoramidite (DMT); 5-Methyl-2'-deoxythymidine, 5'-O-(4,4'-dimethoxytrityl)-; 5'-O-DMT-Thymidine; Thymidine, 5'-O-(bis(4-methoxyphenyl)phenylmethyl)-; dT-CE Phosphoramidite (DMT); 2'-Deoxythymidine 5'-O-(4,4'-dimethoxytrityl) derivative; N-Thyminyl 5'-O-DMT-2'-deoxyriboside; Protected Thymidine for Oligo Synthesis
EINECS	Contact for details

Quality Control

Our production of this critical nucleic acid intermediate adheres to stringent Good Manufacturing Practice (GMP) principles where applicable. Every batch undergoes comprehensive analytical characterization using HPLC, NMR, and Mass Spectrometry to ensure identity, purity, and chiral integrity. We provide detailed Certificates of Analysis (COA) with each shipment, documenting key parameters such as assay purity, moisture content (KF), and levels of critical impurities. Our quality system is designed to meet the exacting standards of pharmaceutical R&D and cGMP starting material requirements.

Storage

Preserve in a tightly closed container, protected from light. This product is hygroscopic (moisture-sensitive). For long-term stability, store desiccated at -20°C or below. Under these conditions, the material is typically stable for 24 months from the date of receipt. Allow the sealed container to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH Q3C guidelines
Specific Rotation	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.