Description

(5'-o-(Dimethoxytrityl)-5-[n-(6-(Trifluoroacetamido)Hexyl)-3-(E)-Acrylamido]-2'-Deoxyuridine) CAS NO 252337-59-0 is a sophisticated, modified nucleoside building block designed for advanced oligonucleotide synthesis. This compound matters for its role in creating functionalized DNA probes and therapeutic oligonucleotides, enabling precise targeting and detection. It is primarily needed by pharmaceutical R&D teams, diagnostic developers, and academic research institutions focused on antisense technology, aptamer development, and high-sensitivity molecular diagnostics.

Application

• Functionalized Oligonucleotide Synthesis: Key monomer for introducing reactive acrylamide groups into DNA strands for post-synthetic conjugation.
• Therapeutic Aptamer Development: Enables the site-specific attachment of payloads (e.g., drugs, fluorophores) to oligonucleotides for targeted therapies.
• Diagnostic Probe Fabrication: Used to create labeled DNA probes for fluorescence in situ hybridization (FISH) and microarray applications.
• Bioconjugation and Cross-linking: The pendant acrylamido group allows for covalent linkage to proteins, surfaces, or other biomolecules.
• Solid-Phase Synthesis: Employed in automated DNA synthesizers, where the dimethoxytrityl (DMT) group serves as a protecting group for the 5'-hydroxyl.
• Research into DNA-Protein Interactions: Serves as a tool to study and map nucleic acid interactions by providing a defined attachment point.

Basic Information

Product Name	(5'-o-(Dimethoxytrityl)-5-[n-(6-(Trifluoroacetamido)Hexyl)-3-(E)-Acrylamido]-2'-Deoxyuridine)
CAS No.	252337-59-0
Molecular Formula	C43H48F3N5O9
Molecular Weight	859.87 g/mol
Synonyms	5'-O-DMT-5-[N-(6-Trifluoroacetamidohexyl)-3-acrylamido]-2'-deoxyuridine; DMT-dU-C6-Acr-TFA Amidite Precursor; 5-(6-Trifluoroacetamidohexylacrylamido)-2'-deoxyuridine, 5'-O-(4,4'-dimethoxytrityl)-; 5-[N-[6-[(Trifluoroacetyl)amino]hexyl]-3-acrylamido]-2'-deoxyuridine 5'-(4,4'-dimethoxytrityl) ether; Acrylamido-modified dU phosphoramidite precursor; Functionalized deoxyuridine derivative; TFA-protected aminohexylacrylamido dU(DMT)
EINECS	Contact for details

Quality Control

Our production of this high-value nucleoside derivative adheres to stringent quality protocols to ensure batch-to-batch consistency and reliability for sensitive research and development applications. Each lot undergoes comprehensive analysis, including HPLC purity verification, identity confirmation by NMR and mass spectrometry, and moisture content analysis. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting all critical parameters to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This material is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, Acetonitrile, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.