Description

Digoxigenin Hydrate is a high-purity, plant-derived cardenolide aglycone widely used as a critical biochemical reagent. Its primary commercial value lies in its role as a highly specific hapten for labeling and detection in molecular biology and diagnostic applications. This compound is essential for researchers and manufacturers in the pharmaceutical, biotechnology, and in-vitro diagnostics (IVD) sectors who require reliable, high-affinity probes for sensitive assay development.

Application

• Molecular Biology Probes: Conjugated to antibodies, nucleotides, or enzymes (e.g., alkaline phosphatase) for use in ELISA, Western blotting, and in-situ hybridization (ISH) for highly sensitive detection.
• Diagnostic Kit Manufacturing: Serves as a key labeling component in the production of immunodiagnostic test kits and biosensors for clinical and research use.
• Pharmaceutical Research: Used as a reference standard and analytical tool in cardiac glycoside research, metabolism studies, and drug discovery programs targeting Na+/K+-ATPase.
• Histochemistry & Cytochemistry: Employed in tissue staining and microscopy applications to visualize specific antigens or nucleic acid sequences with high resolution.
• Bioconjugation & Linker Chemistry: Acts as a versatile hapten for creating custom labeled reagents, including digoxigenin-labeled dUTP for non-radioactive DNA labeling.

Basic Information

Product Name	Digoxigenin Hydrate
CAS No.	207226-38-8
Molecular Formula	C₂₃H₃₄O₆ • xH₂O
Molecular Weight	406.52 g/mol (anhydrous basis)
Synonyms	Digoxigenin monohydrate; (3β,5β,12β)-3,12,14-Trihydroxycard-20(22)-enolide hydrate; Card-20(22)-enolide, 3,12,14-trihydroxy-, (3β,5β,12β)-, hydrate; Digoxigenin; 3β,12β,14-Trihydroxy-5β-card-20(22)-enolide; Lanadigenin; Anhydrogitalin genin
EINECS	Contact for details

Quality Control

Our Digoxigenin Hydrate is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, identity confirmation by IR and NMR, and loss on drying analysis. A Certificate of Analysis (COA) detailing all specifications is provided with every shipment to ensure traceability and compliance with your research or production standards.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. Due to its hygroscopic nature, the container should be kept in a desiccated environment to prevent moisture uptake. For long-term storage, consider storing under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to reference spectrum
Identification (HPLC)	Retention time corresponds to reference standard
Purity (HPLC)	≥ 98.0%
Loss on Drying	≤ 2.0 %
Specific Rotation	Contact for details
Heavy Metals	< 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.