Description

Dmt-Locmec(Bz)Amidite 0.25G 89 Single is a specialized phosphoramidite monomer, a critical building block in automated oligonucleotide synthesis. This high-purity reagent is essential for the precise and efficient assembly of DNA and RNA sequences. It is primarily required by biotechnology, pharmaceutical, and diagnostic research sectors for applications in therapeutic development, genetic analysis, and molecular diagnostics.

Application

• Oligonucleotide Synthesis: As a key phosphoramidite monomer in solid-phase synthesis for research-scale and small-scale production of DNA/RNA strands.
• Therapeutic Development: For the synthesis of antisense oligonucleotides, siRNA, and aptamers used in novel drug discovery and development pipelines.
• Diagnostic Probe Manufacture: In the production of labeled probes and primers for PCR, qPCR, and next-generation sequencing (NGS) applications.
• Academic & Industrial Research: A fundamental reagent for molecular biology research, genetic engineering, and synthetic biology projects.
• CRISPR/Cas9 System Preparation: For synthesizing guide RNAs (gRNAs) and other custom oligonucleotides required for genome editing workflows.

Basic Information

Product Name	Dmt-Locmec(Bz)Amidite 0.25G 89 Single
CAS No.	206055-82-5
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-2'-O-[(benzoyloxy)methyl]uridine 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; DMT-2'-O-(Benzoyloxymethyl)uridine CE Phosphoramidite; 2'-O-BzOM-U Phosphoramidite; 2'-O-(Benzoyloxymethyl)-5'-O-DMT-uridine 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; 2'-O-BzOM-5'-O-DMT-Uridine Phosphoramidite; Amidite monomer for 2'-O-BzOM-U; RNA phosphoramidite building block
EINECS	Contact for details

Quality Control

Our Dmt-Locmec(Bz)Amidite is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive synthesis applications. Each lot undergoes rigorous analytical testing, including HPLC for purity and 31P NMR for structural confirmation, to meet the exacting standards of oligonucleotide synthesis. A comprehensive Certificate of Analysis (COA) detailing purity, moisture content, and performance data is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a freezer at -20°C or below under an inert atmosphere (argon or nitrogen). This product is hygroscopic (moisture-sensitive); allow the sealed vial to equilibrate to room temperature before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (31P NMR)	Conforms to reference standard
Purity (HPLC)	≥ 97.0%
Moisture Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.