Description

1-[5'-o-(4,4'-Dimethoxytrityl)-2-O,4-C-Methylene-β-D-Ribofuranosyl]-5-Methyluracil is a sophisticated nucleoside analog building block, specifically designed for advanced oligonucleotide synthesis. This compound is critical for researchers and manufacturers developing novel therapeutic and diagnostic agents, particularly in the fields of antisense oligonucleotides and siRNA. It is primarily utilized by pharmaceutical R&D departments, biotechnology companies, and fine chemical suppliers focused on nucleic acid chemistry and medicinal chemistry.

Application

• Oligonucleotide Synthesis: A key phosphoramidite precursor for the solid-phase synthesis of modified oligonucleotides, especially those containing locked nucleic acid (LNA) or bridged nucleic acid (BNA) motifs.
• Antisense Drug Development: Used in the research and production of antisense oligonucleotides (ASOs) designed for targeted gene silencing in therapeutic applications.
• siRNA and miRNA Research: Essential for constructing chemically modified small interfering RNA (siRNA) and microRNA (miRNA) strands to enhance stability, specificity, and delivery.
• Diagnostic Probe Manufacture: Employed in creating high-affinity, nuclease-resistant probes for molecular diagnostics, including FISH and PCR applications.
• Medicinal Chemistry & Lead Optimization: Serves as a crucial intermediate for modifying the sugar-phosphate backbone of nucleic acids to improve pharmacokinetic properties.
• Academic & Institutional Research: Supports fundamental studies in nucleic acid structure, function, and the development of novel bio-conjugates.

Basic Information

Product Name	1-[5'-o-(4,4'-Dimethoxytrityl)-2-O,4-C-Methylene-β-D-Ribofuranosyl]-5-Methyluracil
CAS No.	206055-71-2
Molecular Formula	C33H34N2O8
Molecular Weight	586.64 g/mol
Synonyms	5'-O-DMT-2'-O,4'-C-Methylene-5-methyluridine; 5'-O-(4,4'-Dimethoxytrityl)-2'-O,4'-C-methylene-5-methyluridine; DMT-2',4'-BNA(T)-CE Phosphoramidite precursor; 2'-O,4'-C-Methylene-5-methyluridine 5'-O-DMT; LNA-5-Methyluridine DMT protected; BNA-T DMT Derivative; 1-[5'-O-(Bis(4-methoxyphenyl)phenylmethyl]-2-O,4-C-methylene-β-D-ribofuranosyl]-5-methylpyrimidine-2,4(1H,3H)-dione
EINECS	Contact for details

Quality Control

Our production of this high-purity building block adheres to stringent quality protocols to ensure batch-to-batch consistency and reliability for critical synthesis workflows. Certificates of Analysis (COA) are provided with each shipment, detailing purity, identity, and impurity profiles. Quality is verified against in-house specifications developed for nucleic acid synthesis, with testing typically including HPLC purity (≥98%), identity confirmation by spectroscopic methods, and moisture content analysis. We support cGMP-compliant manufacturing for advanced therapeutic applications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This material is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions or a dry inert atmosphere to maintain stability. For long-term storage, consider desiccated conditions at -20°C.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solvent Residue (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.