Description

Poly(Dt-Dg)N.Poly(Dc-Da)N CAS NO 27732-52-1 is a synthetic polynucleotide complex, a high-purity biochemical reagent designed for advanced research and development. This compound is critical for applications requiring specific nucleic acid interactions and serves as a model system in structural and biophysical studies. It is primarily utilized by biotechnology firms, academic research institutions, and pharmaceutical companies engaged in molecular biology, drug discovery, and diagnostic assay development.

Application

• Molecular Biology Research: Used as a template or substrate in enzymatic studies, including investigations into polymerase and nuclease activity.
• Biophysical Analysis: Serves as a model compound for studying nucleic acid duplex stability, hybridization kinetics, and secondary structure formation using techniques like CD spectroscopy and calorimetry.
• Diagnostic Development: Acts as a key component in the design and validation of novel nucleic acid-based detection assays and biosensors.
• Pharmaceutical R&D: Employed in screening platforms to identify and characterize compounds that interact with specific DNA or RNA sequences.
• Standards and Controls: Functions as a well-defined reference material or positive control in quantitative PCR (qPCR) and other amplification-based techniques.
• Academic Teaching: Provides a specialized reagent for advanced coursework in biochemistry and molecular genetics laboratories.

Basic Information

Product Name	Poly(Dt-Dg)N.Poly(Dc-Da)N
CAS No.	27732-52-1
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Poly(deoxythymidylyl-deoxyguanylyl) nucleotide; Poly(dT-dG) · Poly(dC-dA); Poly(dT-dG):Poly(dC-dA); Alternating copolymer d(TG):d(CA); Synthetic DNA duplex d(TG)n·d(CA)n; Polynucleotide complex 27732-52-1; Poly(dT-dG) paired with Poly(dC-dA)
EINECS	Contact for details

Quality Control

Every batch of Poly(Dt-Dg)N.Poly(Dc-Da)N is manufactured under controlled conditions and undergoes rigorous analytical testing to ensure identity, purity, and consistency. Our quality assurance protocol is designed to meet the exacting standards of life science research. A comprehensive Certificate of Analysis (COA) detailing purity, absorbance ratios, and physical state is provided with each shipment to guarantee traceability and compliance with your research requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to reach room temperature before opening to minimize condensation and moisture uptake. For long-term stability, store desiccated.

Specification

Item	Specification
Appearance	White to off-white solid or lyophilized powder
Identification (UV-Vis)	Characteristic absorbance profile consistent with double-stranded DNA
Purity (HPLC)	≥ 95% (by area)
A260/A280 Ratio	1.8 - 2.0
Hypochromicity	≥ 25% (confirms duplex formation)
Solubility	Soluble in TE buffer or nuclease-free water
Biological Activity	Suitable for use as a substrate in enzymatic assays

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.