Description

Poly A-T CAS NO 27156-07-6 is a synthetic polynucleotide composed of alternating adenine and thymine bases. This well-defined oligomer is a critical reagent in molecular biology research, serving as a model substrate and a versatile tool for studying nucleic acid interactions. It is essential for researchers and manufacturers in the biotechnology, pharmaceutical, and diagnostic sectors who require high-purity, sequence-specific nucleic acids for applications such as probe development, hybridization studies, and assay calibration.

Application

• Molecular Biology Research: Used as a standard or control in hybridization experiments, Southern/Northern blotting, and polymerase chain reaction (PCR) optimization.
• Diagnostic Assay Development: Serves as a critical component in the design and validation of diagnostic kits, including those for genetic testing and pathogen detection.
• Biochemical Studies: Employed as a model substrate to investigate enzyme kinetics of nucleases, polymerases, and ligases under controlled conditions.
• Probe Synthesis: Acts as a template or competitor in the synthesis and purification of labeled nucleic acid probes for imaging and detection.
• Biophysical Analysis: Utilized in studies of DNA melting temperature (Tm), duplex stability, and secondary structure formation due to its predictable AT-rich sequence.
• Quality Control Standard: Provides a reference material for verifying the performance and sensitivity of laboratory equipment and analytical methods in genomics workflows.

Basic Information

Product Name	Poly A-T CAS NO 27156-07-6
CAS No.	27156-07-6
Molecular Formula	(C10H12N5O6P • C10H13N2O8P)n
Molecular Weight	Variable (based on chain length 'n')
Synonyms	Poly(deoxyadenylic-deoxythymidylic) acid; Poly(dA-dT); Poly(dA-dT) Homopolymer; Alternating dA-dT Polynucleotide; Poly(deoxyadenylate-thymidylate); DNA, poly(dA-dT); Poly(dAdT); d(pA-pT)n
EINECS	Contact for details

Quality Control

Our Poly A-T is manufactured under stringent conditions to ensure batch-to-batch consistency and high performance. Each lot undergoes comprehensive analytical testing, including HPLC for purity assessment, UV spectroscopy for concentration verification, and gel electrophoresis for chain length integrity. A Certificate of Analysis (COA) detailing purity, absorbance ratios (A260/A280), and endotoxin levels is provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C. This product is hygroscopic (moisture-sensitive). Allow the vial to reach room temperature before opening to prevent condensation and moisture uptake. Aliquot into smaller volumes after initial thawing to avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder or solid
Identification (UV-Vis)	Characteristic absorbance maximum at ~260 nm
Purity (HPLC)	≥ 95% (oligonucleotide content)
Absorbance Ratio (A260/A280)	1.8 - 2.0
Chain Length (n)	Specified range (e.g., 100-500 base pairs)
Endotoxin Level	< 10 EU/mg (for molecular biology grade)
Solubility	Soluble in TE buffer or nuclease-free water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.