Description

5-Bromo-2'-Deoxyuridine-2-14C is a radiolabeled analog of thymidine, where a carbon-14 isotope is incorporated at the 2-position of the pyrimidine ring. This compound is critically important for tracing and quantifying DNA synthesis, replication, and cell proliferation in biochemical and pharmacological research. It is an essential tool for scientists in oncology, virology, and molecular biology who require precise metabolic pathway analysis. The product is supplied with a detailed Certificate of Analysis specifying the exact radioactive concentration and radiochemical purity.

Application

• DNA Synthesis & Cell Proliferation Assays: Used as a tracer to measure the rate of DNA synthesis in cell cultures, a key metric in cancer research and drug discovery.
• Antiviral & Anticancer Drug Development: Employed in studies to evaluate the efficacy of nucleoside analogs by monitoring their incorporation into viral or tumor cell DNA.
• Metabolic Pathway Tracing: Facilitates the study of nucleotide salvage pathways and the metabolic fate of nucleoside analogs in vitro.
• Autoradiography & Molecular Imaging: Serves as a radioactive probe for high-resolution imaging of DNA synthesis locations in tissues or electrophoretic gels.
• Biochemical Mechanism Studies: Essential for investigating enzyme kinetics of thymidine kinase and other nucleoside-metabolizing enzymes.
• Toxicology & Genotoxicity Testing: Used in assays to assess compound-induced DNA damage and repair mechanisms.

Basic Information

Product Name	5-Bromo-2'-Deoxyuridine-2-14C
CAS No.	26291-66-7
Molecular Formula	C9H11Br14CN2O5
Molecular Weight	Contact for details
Synonyms	5-BrdU-2-14C; [2-14C]-5-Bromo-2'-deoxyuridine; 5-Bromo-1-(2-deoxy-β-D-ribofuranosyl)uracil-2-14C; BrdU-2-14C; NSC 38297-2-14C; 5-Bromo-2'-deoxyuridine (2-14C); Radiolabeled BrdU
EINECS	Contact for details

Quality Control

Every batch of 5-Bromo-2'-Deoxyuridine-2-14C is manufactured and analyzed under strict quality systems. Our products undergo rigorous quality testing to ensure compliance with industry standards for radiochemical purity, specific activity, and chemical identity. Certificates of Analysis (COA) are provided with each shipment, detailing results from HPLC, NMR, and mass spectrometry analyses to guarantee traceability and batch-to-batch consistency for your critical research.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C. The product is light-sensitive and should be handled to minimize exposure. For long-term storage, keep frozen at -20°C or below. Please refer to the specific Certificate of Analysis for the recommended storage conditions of your batch.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Radiochemical Purity (HPLC)	≥ 98%
Chemical Purity (HPLC)	≥ 95%
Specific Activity	50-60 mCi/mmol (Contact for exact value)
Solvent (for solution forms)	Ethanol:Water (7:3) or as specified

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.