Description

Deoxycytidine, [5-3H] is a tritium-labeled nucleoside analog essential for advanced biochemical and pharmacological research. This high-specific-activity compound serves as a critical tracer for studying DNA synthesis, repair mechanisms, and nucleoside metabolism in vitro and in vivo. It is primarily utilized by research scientists and pharmaceutical developers in radiochemical synthesis, molecular biology, and oncology drug discovery programs.

Application

• Tracer Studies in DNA Synthesis & Repair: Used to label and track the incorporation of deoxycytidine into DNA, enabling research on cell proliferation, replication fidelity, and DNA damage response pathways.
• Metabolic Pathway Analysis: Employed in pharmacokinetic and metabolic studies to investigate the uptake, activation, and catabolism of nucleoside analogs in biological systems.
• Radiochemical Synthesis: Acts as a labeled precursor for the synthesis of more complex tritiated nucleotides, oligonucleotides, or potential therapeutic agents.
• Oncology & Antiviral Research: Facilitates the study of nucleoside-based chemotherapeutic and antiviral agents, helping to elucidate their mechanisms of action and resistance.
• Enzyme Assay Development: Used as a substrate in assays for enzymes such as deoxycytidine kinase, deaminases, and other nucleoside-metabolizing enzymes.

Basic Information

Product Name	Deoxycytidine, [5-3H]
CAS No.	25352-62-9
Molecular Formula	C9H13N3O4 (³H)
Molecular Weight	Contact for details
Synonyms	2'-Deoxy[5-³H]cytidine; [5-³H]dCyd; Cytosine deoxyriboside, tritiated; 1-(2-Deoxy-β-D-erythro-pentofuranosyl)-4-amino-2(1H)-pyrimidinone, [5-³H]-; 4-Amino-1-(2-deoxy-β-D-ribofuranosyl)-2(1H)-pyrimidinone, [5-³H]-; Tritiated Deoxycytidine; dC([5-³H])
EINECS	Contact for details

Quality Control

Every batch of Deoxycytidine, [5-3H] is manufactured and analyzed under strict quality systems. Radiochemical purity and specific activity are determined by validated methods including HPLC with radiometric detection. A comprehensive Certificate of Analysis (COA) is provided with each shipment, detailing specific activity, purity, solvent composition, and analytical results.

Storage

Preserve in a tightly closed container, protected from light. Store at or below -20°C in the original solvent as supplied. Due to its highly volatile nature (tritium), appropriate containment and laboratory practices for handling radioactive materials must be followed at all times. For long-term storage, keep in a dedicated, well-ventilated area designated for radiochemicals.

Specification

Item	Specification
Appearance	Clear, colorless solution
Specific Activity	10-30 Ci/mmol (Contact for exact value)
Radiochemical Purity (HPLC)	≥ 95%
Chemical Purity (HPLC)	≥ 98%
Identification (HPLC, MS)	Conforms to reference standard
Solvent	Ethanol:Water (Specific ratio per batch)
Concentration	1 mCi/mL (Nominal)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.