Description

Poly I is a synthetic, high-molecular-weight polynucleotide composed of inosinic acid residues. This compound serves as a critical research tool and a potent immunostimulant, primarily functioning as a synthetic analog of double-stranded RNA. It is essential for researchers and manufacturers in the life sciences sector who require a reliable, high-purity inducer of interferon production and immune response modulation. Its applications span advanced pharmaceutical development, immunological research, and bioprocess optimization.

Application

• Immunological Research: Widely used as a standard inducer of interferon (IFN) production in cell culture studies to model viral infection and innate immune responses.
• Vaccine Adjuvant Development: Investigated as a potent adjuvant to enhance the efficacy and longevity of immune responses in next-generation vaccine formulations.
• Oncology Research: Employed in preclinical studies to stimulate anti-tumor immunity and evaluate combination therapies with immunomodulatory agents.
• Antiviral Agent Screening: Serves as a critical control and challenge agent in assays designed to discover and validate novel antiviral compounds.
• Biochemical Standard: Used as a reference standard in molecular biology for studying RNA-protein interactions and Toll-like receptor (TLR) signaling pathways, specifically TLR3.
• Cell Signaling Studies: Applied to activate protein kinase R (PKR) and other dsRNA-sensing pathways to elucidate cellular defense mechanisms.

Basic Information

Product Name	Poly I
CAS No.	25249-22-3
Molecular Formula	(C10H13N4O8P)n
Molecular Weight	Variable (High Polymer)
Synonyms	Polyinosinic acid; Poly(I); Polynosinic acid; Poly I homopolymer; Inosinic acid homopolymer; Polyriboinosinic acid; 9-β-D-Ribofuranosylhypoxanthine homopolymer; TLR3 Agonist (Poly I)
EINECS	Contact for details

Quality Control

Our Poly I is manufactured under strict quality management systems to ensure batch-to-batch consistency and reliability for sensitive research applications. Each lot undergoes comprehensive analytical testing, including assessment of polynucleotide length, purity, and biological activity. A Certificate of Analysis (COA) detailing specifications for identity, assay, endotoxin levels, and bioburden is provided with every shipment to support your quality and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). For long-term storage, desiccate at -20°C. Allow the vial to reach room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white fibrous solid or lyophilized powder
Identification (IR)	Conforms to reference spectrum
Assay (HPLC)	≥ 95% (Poly I content)
Absorbance Ratio (A250/A260)	0.80 - 0.90
Hyperchromicity	≥ 25%
Endotoxin	< 10 EU/mg
Biological Activity (Cell-based assay)	Induces interferon-alpha production in specified cell lines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.