Description

Oligo(Dt)-Oligo(Da) CAS NO 24939-09-1 is a synthetic oligonucleotide duplex, specifically a homopolymer pair of deoxyadenosine (dA) and deoxythymidine (dT). This product is a critical reagent in molecular biology research, serving as a fundamental tool for nucleic acid hybridization studies and calibration. It is essential for researchers and manufacturers in the biotechnology, pharmaceutical, and diagnostic sectors who require high-purity, defined-sequence oligonucleotides for applications such as primer design validation, probe development, and assay standardization.

Application

• Molecular Biology Research: Used as a model system for studying DNA-DNA hybridization kinetics, duplex stability (melting temperature, Tm), and the effects of salt concentration on nucleic acid interactions.
• Diagnostic Assay Development: Serves as a positive control or calibration standard in the development and validation of PCR, qPCR, and other nucleic acid amplification tests (NAATs).
• Biochemical Reagent: Acts as a substrate or competitor in enzymatic assays for polymerases, nucleases, and helicases to assess enzyme activity and specificity.
• Biotechnology Tool: Employed in the development and optimization of biosensors, microarrays, and next-generation sequencing platforms that rely on specific oligonucleotide binding.
• Academic & Teaching Laboratories: Provides a well-characterized, predictable duplex for educational demonstrations of nucleic acid principles and laboratory techniques.

Basic Information

Product Name	Oligo(Dt)-Oligo(Da)
CAS No.	24939-09-1
Molecular Formula	Contact for details
Molecular Weight	Contact for details (varies by chain length)
Synonyms	Poly(dA)-Poly(dT); Poly(deoxyadenylic acid)-Poly(deoxythymidylic acid); dA-dT Oligonucleotide Duplex; Oligo(dA)-Oligo(dT); Homopolymer DNA Duplex; (dA)n-(dT)n; Deoxyadenosine-Thymidine Oligomer Pair; DNA Oligomer Duplex Standard
EINECS	Contact for details

Quality Control

Our Oligo(Dt)-Oligo(Da) is manufactured under stringent conditions to ensure batch-to-batch consistency and high purity, suitable for sensitive research applications. Each lot is characterized to confirm identity, purity, and duplex integrity. Certificates of Analysis (COA) detailing specifications such as HPLC purity, absorbance ratios (A260/A280), and endotoxin levels are available upon request to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a non-frost-free freezer for long-term stability. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature before opening to prevent condensation. For short-term use, store at 2-8°C. Use ultrapure, nuclease-free buffers for reconstitution and aliquoting to prevent degradation.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder or solid
Identification (HPLC/UV)	Conforms
Purity (HPLC)	≥ 95% (Oligonucleotide Duplex)
Absorbance Ratio (A260/A280)	1.8 - 2.0
Duplex Integrity (Melting Curve)	Single, sharp transition
Endotoxin	< 10 EU/mg (if specified for molecular biology grade)
Solubility	Soluble in TE buffer or nuclease-free water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.