Description

5-Methyldeoxycytidine Triphosphate (CAS NO 22003-12-9) is a modified nucleotide triphosphate essential for advanced molecular biology and epigenetics research. This compound serves as a critical substrate for DNA polymerases, enabling the site-specific incorporation of 5-methylcytosine into DNA strands. It is primarily utilized by researchers and developers in pharmaceutical R&D, diagnostic assay development, and synthetic biology for studying DNA methylation patterns, gene expression regulation, and developing novel therapeutic approaches.

Application

• Epigenetics Research: Used as a substrate for in vitro DNA methylation studies and enzymatic synthesis of methylated DNA probes.
• Next-Generation Sequencing (NGS): Employed in the preparation of sequencing libraries to study genome-wide methylation profiles (e.g., bisulfite sequencing workflows).
• PCR Amplification: Facilitates the amplification of methylated DNA sequences for diagnostic and research applications.
• DNA Synthesis & Cloning: Essential for the enzymatic synthesis of oligonucleotides and genes with defined methylation patterns.
• Diagnostic Development: Key component in the development of assays for detecting methylation-based biomarkers associated with diseases like cancer.
• Enzyme Kinetics Studies: Used to study the activity and specificity of DNA methyltransferases and other modifying enzymes.

Basic Information

Product Name	5-Methyldeoxycytidine Triphosphate
CAS No.	22003-12-9
Molecular Formula	C10H17N3O13P3
Molecular Weight	503.17 g/mol
Synonyms	5-Methyl-dCTP; 5-Methyl-2'-deoxycytidine-5'-triphosphate; 5-Methyldeoxycytidine 5'-triphosphate; 5-Methyl-dCytidine triphosphate; 5m-dCTP; 5-Methylcytosine deoxyribonucleoside triphosphate; (2'R)-2'-Deoxy-5-methylcytidine 5'-(tetrahydrogen triphosphate)
EINECS	Contact for details

Quality Control

Our 5-Methyldeoxycytidine Triphosphate is manufactured under stringent quality systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry for identity verification, to ensure it meets the high standards required for sensitive life science applications. A Certificate of Analysis (COA) detailing purity, identity, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. The product is hygroscopic (moisture-sensitive) and should be allowed to equilibrate to room temperature before opening to minimize moisture uptake. For long-term storage, consider storing under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5%
Biological Activity	Suitable for enzymatic incorporation

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.