Description

Biotin-PEG12-Acid is a high-purity, heterobifunctional polyethylene glycol (PEG) linker featuring a biotin moiety for affinity capture and a terminal carboxylic acid for conjugation. This compound is essential for creating stable, water-soluble bioconjugates with precise spacing and reduced steric hindrance. It is primarily used by researchers and manufacturers in pharmaceutical development, diagnostics, and advanced materials science for applications such as drug delivery systems, surface functionalization, and assay development.

Application

• ADC (Antibody-Drug Conjugate) Linker: Serves as a cleavable or non-cleavable spacer to connect cytotoxic payloads to monoclonal antibodies, enhancing solubility and pharmacokinetics.
• Diagnostic Probe Conjugation: Used to attach biotin labels to proteins, peptides, or oligonucleotides for detection in ELISA, Western blot, or lateral flow assays via streptavidin binding.
• Surface Modification & Biotinylation: Functionalizes nanoparticles, microspheres, or biosensor surfaces with a carboxylic acid handle for further coupling, followed by biotin-mediated immobilization.
• Drug Delivery System Fabrication: Incorporated into liposomes, dendrimers, or polymeric micelles to create targeted delivery platforms with enhanced circulation time.
• Affinity Chromatography Ligand: Immobilized onto resin beads via the acid group to create media for the purification of streptavidin/avidin-tagged biomolecules.
• Crosslinking Reagent: Acts as a heterobifunctional crosslinker for conjugating amine-containing molecules (via the acid) to avidin/biotin-binding proteins.
• Biomaterial Engineering: Integrates into hydrogels or scaffolds to introduce bioactive biotin sites for controlled cell adhesion or growth factor presentation.
• Chemical Biology Tool: Enables the study of protein-protein interactions, cellular trafficking, and biomolecular localization through site-specific biotin tagging.

Basic Information

Item	Details
Product Name	Biotin-PEG12-Acid
CAS No.	1621423-14-0
Molecular Formula	C53H100N4O27S
Molecular Weight	1281.43 g/mol
Synonyms	Biotin-PEG12-COOH; Biotin-PEG12-COOH; Biotin-PEG12-Acid; Biotin-PEG12-Propionic Acid; Biotinylated PEG12 Acid; 5-[(3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]-N-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-(2-carboxyethoxy)ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethyl]pentanamide; Biotin-PEG12-Propanoic Acid; Biotin-12-EG-COOH
EINECS	Contact for details

Quality Control

Our Biotin-PEG12-Acid is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry for identity verification, to ensure it meets the high standards required for research and bioconjugation. Certificates of Analysis (COA) with detailed chromatographic data are provided with every shipment. We support compliance with cGMP, ISO 9001, and REACH frameworks for global supply.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture absorption. For long-term storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (HPLC-MS)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Molecular Weight Verification (MS)	1281.43 ± 2 Da
Functional Group Assay (Acid Titration)	≥ 90%
Biotin Content (UV/HPLC)	Conforms

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.