Description

N4-Acetyl-5'-o-Dmt-2'-o-Propynylcytidine 3'-Ce Phosphoramidite is a high-purity, modified nucleoside phosphoramidite monomer, a critical building block for the solid-phase synthesis of oligonucleotides. This compound is specifically engineered to introduce a propynyl group at the 2'-O position, which is valuable for enhancing the binding affinity and nuclease resistance of synthetic oligonucleotides. It is an essential raw material for research and development in therapeutic oligonucleotide production, diagnostic probe development, and advanced genetic research.

Application

• Therapeutic Oligonucleotide Synthesis: Key monomer for manufacturing antisense oligonucleotides (ASOs), siRNA, and aptamers with enhanced stability.
• Diagnostic Probe Fabrication: Used in the synthesis of labeled probes for fluorescence in situ hybridization (FISH) and microarray technologies.
• Basic Genetic Research: Enables the study of RNA structure and function through the incorporation of modified bases into synthetic strands.
• Antisense Technology Development: Critical for creating research-grade oligonucleotides targeting specific mRNA sequences.
• Pharmaceutical R&D: Serves as a starting material in the development pipeline for novel nucleic acid-based therapeutics.
• Bioconjugation and Labeling: The propynyl group provides a handle for further chemical modifications, such as click chemistry conjugation with fluorescent dyes or other functional groups.

Basic Information

Item	Detail
Product Name	N4-Acetyl-5'-o-Dmt-2'-o-Propynylcytidine 3'-Ce Phosphoramidite
CAS No.	1498305-51-3
Molecular Formula	C46H51N4O9P
Molecular Weight	834.90 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-2'-O-propargyl-N4-acetylcytidine 3'-(2-cyanoethyl N,N-diisopropylphosphoramidite); 2'-O-Propargyl-5'-O-DMT-N4-acetylcytidine 3'-CE phosphoramidite; DMT-rC(ac)-Pr-CE Phosphoramidite; 2'-O-Propynyl-5'-O-DMT-N-acetylcytidine 3'-(2-cyanoethyl N,N-diisopropylphosphoramidite); N4-Acetyl-2'-O-propargyl-5'-O-(4,4'-dimethoxytrityl)cytidine 3'-(2-cyanoethyl N,N-diisopropylphosphoramidite); 2'-O-Propargyl-N4-acetyl-5'-O-DMT-cytidine 3'-CE phosphoramidite
EINECS	Contact for details

Quality Control

Our N4-Acetyl-5'-o-Dmt-2'-o-Propynylcytidine 3'-Ce Phosphoramidite is manufactured under strict quality systems to ensure batch-to-batch consistency for critical oligonucleotide synthesis. Each lot undergoes comprehensive analytical testing, including HPLC purity analysis, 31P and 1H NMR spectroscopy, and moisture content determination. Certificates of Analysis (COA) detailing all specifications are provided to guarantee compliance with your research or cGMP starting material requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C ± 5°C under an inert atmosphere (argon or nitrogen) in a dry, moisture-free environment. This product is hygroscopic (moisture-sensitive) and easily oxidized; prolonged exposure to air or moisture will reduce its performance and shelf life. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Solubility	Soluble in acetonitrile, dichloromethane

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.