Description

5-(3-Azidopropyl)-UTP 4TEA is a chemically modified nucleotide triphosphate analog, featuring an azidopropyl linker at the 5-position of the uridine base. This functionalization is critical for enabling bioorthogonal "click chemistry" reactions, primarily copper-catalyzed azide-alkyne cycloaddition (CuAAC), for the site-specific labeling and conjugation of nucleic acids. It is an essential reagent for researchers and developers in the fields of molecular biology, diagnostics, and chemical biology who require precise tagging of RNA molecules. The tetraethylammonium (TEA) salt form enhances solubility in aqueous buffers, making it suitable for enzymatic incorporation.

Application

• RNA Labeling & Detection: Enzymatic incorporation by RNA polymerases (e.g., T7, SP6) for the post-transcriptional, site-specific attachment of fluorophores, biotin, or other probes via click chemistry.
• Probe Synthesis: Manufacturing of highly specific, covalently labeled RNA probes for techniques such as FISH (Fluorescence In Situ Hybridization), Northern blotting, and microarray analysis.
• Chemical Biology Research: Tool for studying RNA structure, function, localization, and protein-RNA interactions through bioorthogonal tagging strategies.
• Diagnostic Assay Development: Key component in the creation of next-generation diagnostic platforms, including biosensors and assays that require stable, covalent attachment of signal-generating molecules to RNA.
• RNA Therapeutics Conjugation: Research into the conjugation of therapeutic payloads (e.g., drugs, targeting ligands) to RNA-based therapeutics, such as mRNA or aptamers.
• Materials Science: Functionalization of surfaces or nanomaterials with RNA strands for biosensing and nano-construction applications.

Basic Information

Product Name	5-(3-Azidopropyl)-UTP 4TEA
CAS No.	1354419-13-8
Molecular Formula	C₂₀H₃₃N₇O₁₅P₃ • (C₈H₂₀N)₄
Molecular Weight	Contact for details
Synonyms	5-(3-Azidopropyl)uridine-5'-triphosphate tetraethylammonium salt; 5-N₃-propyl-UTP; Azido-UTP; 5-(3-Azidopropyl)-UTP, TEA salt; 5-Azidopropyl-UTP; UTP-azide; 5-AP-UTP; Click-chemistry UTP
EINECS	Contact for details

Quality Control

Our 5-(3-Azidopropyl)-UTP 4TEA is manufactured under strict quality protocols to ensure high purity and batch-to-batch consistency, suitable for sensitive biochemical applications. Each lot is characterized by HPLC, NMR, and MS to confirm identity, purity, and the integrity of the azido functional group. A comprehensive Certificate of Analysis (COA) detailing purity, concentration, and solvent content is provided with every shipment. We support cGMP and ISO 9001 standards for research and diagnostic-grade materials.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a non-frost-free freezer. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a dry environment before opening to prevent condensation and hydrolysis. For long-term storage, consider desiccant and under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid or lyophilized powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5%
Solvent Content (Residual)	Meets ICH guidelines
Functional Integrity (Azide Assay)	Confirmed
Enzymatic Incorporation Test	Suitable for in vitro transcription

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.