Description

Rev 2'-o-Moe-5Meu-5'-Amidite CAS NO 1345715-43-6 is a critical phosphoramidite monomer used in the solid-phase synthesis of oligonucleotides. This high-purity building block is essential for introducing 2'-O-methoxyethyl (2'-MOE) and 5-methyluridine (5-MeU) modifications, which are key to enhancing the stability, binding affinity, and pharmacokinetic properties of therapeutic nucleic acids. It is a vital raw material for pharmaceutical R&D and manufacturing, particularly in the development of antisense oligonucleotides, siRNA, and other advanced genetic medicines.

Application

• Antisense Oligonucleotide (ASO) Synthesis: Primary building block for creating therapeutic ASOs with improved nuclease resistance and target affinity.
• siRNA and miRNA Therapeutics: Used in the synthesis of chemically modified siRNA strands to enhance stability and reduce off-target effects.
• Research-Grade Oligo Synthesis: Enables the production of modified oligonucleotides for basic research, diagnostic probe development, and target validation studies.
• Pharmaceutical Development: Serves as a key starting material (KSM) or intermediate in the GMP-compliant production of oligonucleotide active pharmaceutical ingredients (APIs).
• Synthesis of Aptamers: Facilitates the creation of nuclease-resistant aptamers for therapeutic and diagnostic applications.
• Synthesis of Primers and Probes: Used to manufacture specialized, modified primers and probes for sensitive molecular diagnostics and PCR applications.

Basic Information

Product Name	Rev 2'-o-Moe-5Meu-5'-Amidite
CAS No.	1345715-43-6
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-2'-O-methoxyethyl-5-methyluridine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; 5-MeU 2'-MOE Phosphoramidite; 2'-O-MOE-5-methyluridine Phosphoramidite; 5-Methyl-2'-O-methoxyethyluridine 3'-CE Phosphoramidite; 5-Me-2'-MOE-U Phosphoramidite; 2'-MOE-5-MeU Phosphoramidite; 5-Methyluridine, 2'-O-(2-methoxyethyl)-, 3'-[2-cyanoethyl bis(1-methylethyl)phosphoramidite], 5'-[bis(4-methoxyphenyl)phenylmethyl] ether
EINECS	Contact for details

Quality Control

Our Rev 2'-o-Moe-5Meu-5'-Amidite is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity, essential for sensitive oligonucleotide synthesis. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation (¹H NMR, ³¹P NMR). We provide detailed Certificates of Analysis (COA) with all shipments, documenting critical parameters such as assay, moisture content (Karl Fischer), and purity profile. Our quality commitment supports applications from research to cGMP manufacturing.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C ± 5°C under an inert atmosphere (argon or nitrogen) in a dedicated freezer. This product is hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation. Once opened, use immediately or re-seal under inert gas.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure (¹H, ³¹P)
Purity (HPLC)	≥ 97.0%
Moisture (Karl Fischer)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.