Description

n-Biotinylcaproylaminocaproylaminocaproyl n-Hydroxysuccinimide is a specialized, long-chain biotinylation reagent designed for the covalent attachment of biotin to amino groups in biomolecules. This compound matters for its extended spacer arm, which significantly reduces steric hindrance and enhances accessibility for streptavidin/avidin binding, a critical factor in sensitive detection assays. It is primarily needed by researchers and manufacturers in the fields of bioconjugation, diagnostic kit development, and proteomics who require high-efficiency labeling of proteins, antibodies, and other amines.

Application

• Bioconjugation of Proteins and Antibodies: For labeling primary amines (lysine residues) with biotin for use in ELISA, Western blotting, and flow cytometry.
• Diagnostic Assay Development: As a key reagent in the manufacture of lateral flow tests and other immunoassays that utilize streptavidin-biotin technology.
• Affinity Purification Probes: Creation of biotinylated ligands or capture molecules for pull-down assays and mass spectrometry sample preparation.
• Cell Surface Labeling: For tagging membrane proteins on live or fixed cells for imaging and sorting applications.
• Microarray and Chip Fabrication: Immobilization of biomolecules onto streptavidin-coated surfaces for high-throughput screening.
• Nucleic Acid Probes: Biotinylation of amine-modified oligonucleotides for in situ hybridization and detection.

Basic Information

Product Name	n-Biotinylcaproylaminocaproylaminocaproyl n-Hydroxysuccinimide
CAS No.	1322625-23-9
Molecular Formula	C34H58N6O8S
Molecular Weight	710.93 g/mol
Synonyms	Biotin-LC-LC-LC-NHS Ester; Biotin-XX-X SE; Biotin-(AC5)3-OSu; EZ-Link™ Biotin-XX-X, SE (common trade name); N-(+)-Biotinyl-6-aminocaproyl-6-aminocaproyl-6-aminocaproic acid N-hydroxysuccinimide ester; Long-chain Biotin NHS Ester; Biotin with triple aminocaproyl spacer NHS ester; Biotin-XX-X, Sulfosuccinimidyl Ester (water-soluble analog)
EINECS	Contact for details

Quality Control

Our n-Biotinylcaproylaminocaproylaminocaproyl n-Hydroxysuccinimide is manufactured under strict quality protocols to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot undergoes rigorous analytical testing, including HPLC for purity and identity confirmation. Certificates of Analysis (COA) detailing all specifications are available upon request to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and should be allowed to equilibrate to room temperature in a dry environment before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Solubility	Soluble in DMSO, DMF, acetonitrile
Water Content (KF)	≤ 1.0%
Functional Activity	Conforms (by biotinylation test)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.