Description

Fmoc-5’-Amino-Modifier-5 Cep is a specialized phosphoramidite monomer used for the solid-phase synthesis of oligonucleotides. This compound is critical for introducing a primary amine functional group at the 5'-terminus of DNA or RNA strands, enabling subsequent conjugation to dyes, quenchers, or other molecules. It is an essential reagent for researchers and manufacturers in the fields of therapeutic oligonucleotide development, diagnostic probe synthesis, and advanced genomic research.

Application

• Therapeutic Oligonucleotide Synthesis: Key building block for creating antisense oligonucleotides (ASOs), siRNA, and aptamers with 5'-terminal modifications for enhanced targeting or stability.
• Diagnostic Probe Manufacturing: Used to produce labeled DNA/RNA probes for fluorescence in situ hybridization (FISH), microarrays, and real-time PCR applications.
• Bioconjugation Platform: The introduced 5'-amino group serves as a handle for coupling to fluorophores, biotin, peptides, or solid surfaces.
• Next-Generation Sequencing (NGS): Fabrication of custom primers and adapters with unique functional groups for library preparation.
• Materials Science: Synthesis of DNA-functionalized nanomaterials and surfaces for biosensing and nano-assembly.
• Academic & Industrial R&D: Fundamental research tool for exploring nucleic acid chemistry and developing novel bio-conjugates.

Basic Information

Item	Detail
Product Name	Fmoc-5’-Amino-Modifier-5 Cep
CAS No.	1306615-52-0
Molecular Formula	C41H49N4O7P
Molecular Weight	740.83 g/mol
Synonyms	5'-Amino-Modifier C7 Phosphoramidite, Fmoc protected; 1-Dimethoxytrityloxy-3-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite-2-[(9H-fluoren-9-ylmethoxycarbonyl)-amino]-propane-3,3-diol; 5'-Amino-Modifier C7; 5'-Amino Modifier C7 CE Phosphoramidite; 9-Fluorenylmethyl N-[3-[[bis(1-methylethyl)amino]-(2-cyanoethoxy)phosphino]oxy-2-[(4,4'-dimethoxytrityl)oxy]propyl]carbamate
EINECS	Contact for details

Quality Control

Our Fmoc-5’-Amino-Modifier-5 Cep is manufactured under strict quality systems to ensure high purity and batch-to-batch consistency for sensitive oligonucleotide synthesis. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and moisture content. We support compliance with relevant GMP and ISO 9001 standards for research and production use.

Storage

Preserve in a tightly closed container, protected from light. Store at -15°C to -25°C under an inert atmosphere (argon or nitrogen) in a dry, moisture-free environment. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC/MS, 31P NMR)	Conforms to structure
Purity (HPLC)	≥ 97.0%
Water Content (KF)	≤ 0.5%
Solubility	Soluble in anhydrous acetonitrile, dichloromethane

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.