Description

Biotin-11-dUTP is a biotin-labeled deoxyuridine triphosphate nucleotide analog, a critical reagent for non-radioactive nucleic acid labeling and detection. This compound is engineered to enable high-sensitivity detection through its strong interaction with streptavidin or avidin conjugates. It is essential for researchers and diagnostic developers in molecular biology, genomics, and in situ hybridization who require precise, stable, and efficient tagging of DNA probes.

Application

• Non-radioactive DNA labeling for Southern, Northern, and dot/slot blot hybridizations.
• Preparation of biotinylated probes for fluorescence in situ hybridization (FISH) and chromosome painting.
• Nick translation and random priming reactions to generate labeled DNA for microarray analysis.
• Incorporation via PCR or enzymatic synthesis for next-generation sequencing (NGS) library preparation and capture.
• Development of diagnostic assays and biosensors utilizing streptavidin-biotin signal amplification.
• Labeling of oligonucleotides for pull-down assays, chromatin immunoprecipitation (ChIP), and other molecular interaction studies.

Basic Information

Product Name	Biotin-11-dUTP
CAS No.	1221498-88-9
Molecular Formula	C₃₅H₅₀N₆O₁₉P₃
Molecular Weight	990.73 g/mol
Synonyms	Biotin-11-2'-deoxyuridine-5'-triphosphate; Biotin-11-2'-dUTP; 5-(Biotin-11-amidocaproylamidopropyl)-2'-deoxyuridine-5'-triphosphate; Biotinylated dUTP; Biotin-11-dUTP, trisodium salt; dUTP-Biotin; Biotin-11-AA-dUTP; 2'-Deoxyuridine-5'-triphosphate, N-(6-((6-((biotinoyl)amino)hexanoyl)amino)hexyl) derivative
EINECS	Contact for details

Quality Control

Our Biotin-11-dUTP is manufactured under strict quality systems to ensure batch-to-batch consistency and performance in sensitive applications. Each lot is analyzed for critical parameters including purity, labeling efficiency, and absence of contaminating nucleases. A comprehensive Certificate of Analysis (COA) with HPLC, UV-Vis, and functional assay data is provided to guarantee it meets the stringent requirements for life science research and in vitro diagnostic use.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a non-frost-free freezer. The product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature before opening to prevent condensation. For long-term storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid or solution
Identification (HPLC/UV)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Absorbance Ratio (A260/A280)	1.7 - 2.0
Water Content (Karl Fischer)	≤ 5.0%
Functional Test (Enzymatic Incorporation)	≥ 90% incorporation efficiency
Biological Activity (Streptavidin Binding)	Conforms
Nuclease Activity	Not detected

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.