Description

3'-o-Amino-2'-Deoxycytidine 5'-Triphosphate is a chemically modified nucleotide triphosphate analog, a critical building block for advanced molecular biology and diagnostic applications. Its primary value lies in its amino-modified ribose moiety, which provides a reactive handle for conjugation with labels, probes, or other functional groups, enabling the synthesis of custom oligonucleotides. This compound is essential for researchers and developers in the fields of next-generation sequencing (NGS), probe development, and therapeutic nucleic acid research, where precise chemical functionality is required.

Application

• Next-Generation Sequencing (NGS): Used as a substrate for DNA polymerases in sequencing-by-synthesis platforms to introduce specific, labeled nucleotides.
• Fluorescent Probe Synthesis: The amino group serves as a conjugation site for attaching fluorophores (e.g., Cy3, Cy5, FAM) for FISH probes or real-time PCR applications.
• Therapeutic Oligonucleotide Development: Incorporation into antisense oligonucleotides (ASOs), siRNA, or aptamers to modify stability, targeting, or pharmacokinetic properties.
• DNA Microarray and Chip Fabrication: Enables the site-specific attachment of oligonucleotides to solid surfaces for high-throughput genomic analysis.
• Enzymatic DNA Labeling: Facilitates the enzymatic incorporation of biotin, digoxigenin, or other haptens for sensitive detection in blotting and imaging.
• Structural Biology Studies: Used to introduce modifications for cross-linking experiments or to study protein-nucleic acid interactions.

Basic Information

Product Name	3'-o-Amino-2'-Deoxycytidine 5'-Triphosphate
CAS No.	1220515-75-2
Molecular Formula	C9H16N4O13P3
Molecular Weight	505.16 g/mol
Synonyms	3'-Amino-2'-deoxycytidine-5'-triphosphate; 3'-NH2-dCTP; Amino-modified dCTP; 2'-Deoxy-3'-aminocytidine-5'-triphosphate; 3'-Amino-dCTP; 3'-Amino-2'-deoxy-CTP; dCTP-3'-NH2; 3'-Amino-2'-deoxycytidine triphosphate
EINECS	Contact for details

Quality Control

Our 3'-o-Amino-2'-Deoxycytidine 5'-Triphosphate is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity, suitable for sensitive research and diagnostic applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to ISO 9001 quality management principles, and our production environment is designed to meet the exacting standards required for molecular biology-grade reagents.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a non-frost-free freezer. This product is hygroscopic (moisture-sensitive). For long-term storage, it is recommended to store desiccated under an inert atmosphere. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Assay (Enzymatic)	≥ 90% (as triphosphate)
Water Content (KF)	≤ 5.0%
pH (in solution)	6.0 - 7.5
Endotoxin	< 0.1 EU/µmol
Filter Sterilization	0.2 µm filtered (optional)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.