Description

1-Dimethoxytrityloxy-2-(n-Biotinyl-4-Aminobutyl)-Propyl-3-o-(2-Cyanoethyl)-(N,n-Diisopropyl)-Phosphoramidite is a highly specialized, biotinylated phosphoramidite monomer essential for the solid-phase synthesis of oligonucleotide probes. This compound matters because it enables the site-specific introduction of biotin, a critical tag for detection, purification, and immobilization in advanced molecular biology and diagnostic applications. It is primarily needed by research institutions, diagnostic kit manufacturers, and biotechnology companies engaged in nucleic acid research, next-generation sequencing (NGS) probe development, and microarray fabrication.

Application

• Synthesis of Biotin-Labeled Oligonucleotides: For use as probes in hybridization assays like Southern, Northern, and dot blots.
• Next-Generation Sequencing (NGS): Preparation of biotinylated capture probes for target enrichment and library preparation.
• Microarray and Biochip Fabrication: Creating oligonucleotide arrays with biotin handles for streptavidin-based surface attachment.
• Diagnostic Assay Development: Key building block for probes in PCR, qPCR, and isothermal amplification-based diagnostic tests.
• Affinity Purification & Pull-Down Assays: Generating baits for the isolation of proteins, nucleic acids, or other biomolecules via streptavidin-biotin interaction.
• Fluorescence In Situ Hybridization (FISH): Probe synthesis for cytogenetic and pathological analysis.
• Antisense and siRNA Research: Introducing biotin tags into therapeutic oligonucleotides for tracking and mechanistic studies.
• Basic Life Science Research: A versatile tool for any application requiring highly specific, non-radioactive labeling of synthetic DNA or RNA.

Basic Information

Product Name	1-Dimethoxytrityloxy-2-(n-Biotinyl-4-Aminobutyl)-Propyl-3-o-(2-Cyanoethyl)-(N,n-Diisopropyl)-Phosphoramidite
CAS No.	1217500-22-5
Molecular Formula	C58H81N6O9PS
Molecular Weight	1069.34 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-N2-(biotinyl-4-aminobutyl)-2'-deoxyguanosine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; Biotin-dG Phosphoramidite; N2-(Biotin-4-amino-butyl)-5'-O-DMT-2'-deoxyguanosine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; Biotinylated dG CE Phosphoramidite; 1-Dimethoxytrityloxy-2-(N-Biotinyl-4-aminobutyl)-propyl-3-O-(2-cyanoethyl)-(N,N-diisopropyl)-phosphoramidite; Biotin C4 dG Phosphoramidite
EINECS	Contact for details

Quality Control

Our 1-Dimethoxytrityloxy-2-(n-Biotinyl-4-Aminobutyl)-Propyl-3-o-(2-Cyanoethyl)-(N,n-Diisopropyl)-Phosphoramidite is manufactured under strict quality systems suitable for research and diagnostic applications. Each batch undergoes rigorous analytical testing, including HPLC for purity, ³¹P NMR and MS for structural confirmation, and moisture analysis to ensure integrity. Certificates of Analysis (COA) with full chromatographic data are provided to guarantee consistent performance in automated oligonucleotide synthesis.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below under an inert atmosphere (argon or nitrogen) to maintain stability. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Retention time corresponds to reference standard
Identification (³¹P NMR)	Spectrum corresponds to reference standard
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.