Description

6-Fluorescein Dt Phosphoramidite is a specialized phosphoramidite monomer used for the solid-phase synthesis of oligonucleotides. This compound is critical for introducing a fluorescein dye label at the 5'-terminus of DNA or RNA strands, enabling detection and analysis. It is an essential reagent for researchers and manufacturers in molecular diagnostics, genomics, and pharmaceutical development who require high-purity, reliable labeling for probes and primers.

Application

• Fluorescent Probe Synthesis: Primary reagent for the 5'-end labeling of oligonucleotides with fluorescein for use as hybridization probes in FISH (Fluorescence In Situ Hybridization) and other detection assays.
• Molecular Diagnostics: Manufacture of labeled primers and probes for real-time PCR (qPCR), SNP detection, and other diagnostic applications requiring high-sensitivity fluorescence detection.
• Genomics Research: Synthesis of fluorescently-tagged oligonucleotides for sequencing, genotyping, microarray fabrication, and other high-throughput analysis techniques.
• Therapeutic Oligonucleotide Development: Labeling of antisense oligonucleotides, siRNA, and aptamers during research and development stages for pharmacokinetic and biodistribution studies.
• Bio-conjugation & Sensor Development: Used to create fluorescent DNA strands for attachment to nanoparticles, surfaces, or other molecules in biosensor and nanotechnology research.

Basic Information

Product Name	6-Fluorescein Dt Phosphoramidite
CAS No.	1194507-30-6
Molecular Formula	C46H49N4O9P
Molecular Weight	832.88 g/mol
Synonyms	6-FAM Phosphoramidite; 6-Carboxyfluorescein Phosphoramidite; 5'-Fluorescein Phosphoramidite; 6-FAM-CE Phosphoramidite; Fluorescein-dT Phosphoramidite; 6-Fluorescein Amidite; Dye-labeled Phosphoramidite; 6-FAM CE Phosphoramidite (6-Carboxyfluorescein)
EINECS	Contact for details

Quality Control

Our 6-Fluorescein Dt Phosphoramidite is manufactured under strict quality systems to ensure consistency and reliability for automated DNA synthesis. Each batch is subjected to rigorous analytical testing including HPLC, 1H/31P NMR, and mass spectrometry to confirm identity, purity, and performance. Certificates of Analysis (COA) with full chromatographic data are provided to guarantee it meets the stringent requirements for oligonucleotide synthesis and diagnostic applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a desiccated environment. This product is hygroscopic (moisture-sensitive) and easily oxidized. Allow the sealed vial to equilibrate to room temperature before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	Yellow to orange solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.