Description

5'-O-DMT-N6-Acetyl-2'-Deoxyadenosine 3'-CE Phosphoramidite is a high-purity, protected nucleoside phosphoramidite monomer essential for automated oligonucleotide synthesis. This compound is critical for ensuring high coupling efficiency and sequence fidelity in the production of DNA strands. It is primarily required by biotechnology, pharmaceutical, and diagnostic research sectors for applications in antisense therapy, PCR primers, probes, and gene synthesis.

Application

• Solid-Phase Oligonucleotide Synthesis: As a key building block (dA monomer) in automated DNA synthesizers.
• Therapeutic Oligonucleotide Manufacturing: For the production of antisense oligonucleotides (ASOs), siRNA, and aptamers for drug development.
• Research Reagent: Synthesis of custom DNA primers and probes for PCR, qPCR, sequencing, and molecular diagnostics.
• Gene Synthesis and Cloning: Construction of long DNA fragments and synthetic genes.
• Diagnostic Kit Production: Manufacturing of labeled probes for in-situ hybridization (ISH) and microarray technologies.
• Development of DNA-based Nanomaterials: For use in nanotechnology and biodetection systems.

Basic Information

Product Name	5'-O-DMT-N6-Acetyl-2'-Deoxyadenosine 3'-CE Phosphoramidite
CAS No.	1027734-01-5
Molecular Formula	C43H55N6O8P
Molecular Weight	814.92 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-N6-acetyl-2'-deoxyadenosine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; dA-CE Phosphoramidite (Ac); N6-Ac-dA Phosphoramidite; 2'-Deoxyadenosine, 5'-O-[bis(4-methoxyphenyl)phenylmethyl]-N-[bis(1-methylethyl)amino](2-cyanoethoxy)phosphino]-, 6-acetate; DMT-dA(Ac)-CE Phosphoramidite; Ac-dA Phosphoramidite
EINECS	Contact for details

Quality Control

Our phosphoramidites are manufactured under strict quality control in a controlled environment to ensure batch-to-batch consistency and high purity, suitable for critical synthetic applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, moisture content, and functional performance. We support compliance with ISO 9001 quality management standards and relevant guidelines for materials intended for research and pharmaceutical development.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a dry, moisture-free environment. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation and hydrolysis. For long-term stability, store desiccated.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 98.0%
Moisture Content (Karl Fischer)	≤ 0.5%
Solubility	Clear, colorless solution in anhydrous acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.