Description

Pyrene Phosphoramidite Du is a specialized phosphoramidite monomer derivative, a key building block in oligonucleotide synthesis. This compound matters for its role in introducing the highly fluorescent pyrene moiety into nucleic acid strands, enabling advanced research and diagnostic applications. It is essential for researchers and manufacturers in the fields of molecular diagnostics, fluorescence-based sensing, and structural biology who require high-purity, reliable reagents for probe and primer development.

Application

• Fluorescent DNA/RNA Probe Synthesis: Incorporation into oligonucleotides to create highly sensitive fluorescent probes for hybridization assays (e.g., FISH, real-time PCR).
• Molecular Beacon Development: Used as a fluorogenic component in the design of molecular beacons for specific nucleic acid sequence detection.
• Structural Studies of Nucleic Acids: Acts as a fluorescent reporter to study DNA/RNA folding, protein-nucleic acid interactions, and conformational changes.
• Development of Aptamer-Based Sensors: Essential for constructing signaling aptamers (aptasensors) used in medical diagnostics and environmental monitoring.
• Site-Specific Labeling of Oligonucleotides: Enables precise, internal labeling of synthetic oligonucleotides for advanced biophysical and single-molecule studies.
• Research Tool for SNP Detection: Facilitates the development of assays for single nucleotide polymorphism (SNP) analysis due to its environment-sensitive fluorescence.

Basic Information

Product Name	Pyrene Phosphoramidite Du
CAS No.	199920-17-7
Molecular Formula	C41H45N2O5P
Molecular Weight	676.78 g/mol
Synonyms	1-Pyrenemethyl N,N-diisopropylphosphoramidite; 1-Pyrenemethylphosphoramidite diisopropylamine salt; Pyrene-C1-phosphoramidite; Pyrenylmethyl phosphoramidite; 1-Pyrenylmethyl N,N-Diisopropylphosphoramidite; Du Phosphoramidite (Pyrene); 1-Pyrenylmethylphosphoramidite; 1-Pyrenemethyl N,N-Diisopropylphosphoramidite
EINECS	Contact for details

Quality Control

Our Pyrene Phosphoramidite Du is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity for sensitive oligonucleotide synthesis. Each lot undergoes comprehensive analytical testing, including HPLC, NMR, and mass spectrometry, to confirm identity, purity, and absence of critical impurities. Certificates of Analysis (COA) with full specification data are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store under an inert atmosphere (argon or nitrogen) at -20°C or below in a desiccated environment to prevent degradation due to moisture and oxidation. Allow the sealed vial to equilibrate to room temperature before opening to minimize condensation.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.