Description

5-(E-2-Carbomethoxyvinyl)-5'-o-(4,4'-Dimethoxytrityl)-2'-Deoxyuridine, 3'-[(2-Cyanoethyl)-(N,n-Diisopropyl)]Phosphoramidite is a high-purity, specialty phosphoramidite monomer essential for the solid-phase synthesis of oligonucleotides. This compound is critical for introducing modified nucleosides into DNA strands, enabling advanced research and therapeutic development. It is primarily used by biotechnology and pharmaceutical companies engaged in antisense therapy, diagnostic probe development, and foundational genomic research.

Application

• Solid-Phase Oligonucleotide Synthesis: Key building block for automated DNA/RNA synthesizers to incorporate 5-modified deoxyuridine derivatives.
• Antisense Oligonucleotide (ASO) Production: Synthesis of therapeutic oligonucleotides for gene silencing and targeted drug development.
• Diagnostic Probe Manufacture: Creation of labeled probes for fluorescence in situ hybridization (FISH) and other molecular diagnostic assays.
• Research-Grade Oligo Synthesis: Enables academic and industrial research into nucleic acid chemistry, structure, and function.
• Synthesis of Modified DNA Libraries: For SELEX (Systematic Evolution of Ligands by EXponential enrichment) and aptamer development.
• Preparation of Conjugated Oligonucleotides: Facilitating the attachment of reporter molecules or other functional groups via the carbomethoxyvinyl moiety.

Basic Information

Product Name	5-(E-2-Carbomethoxyvinyl)-5'-o-(4,4'-Dimethoxytrityl)-2'-Deoxyuridine, 3'-[(2-Cyanoethyl)-(N,n-Diisopropyl)]Phosphoramidite
CAS No.	198080-38-5
Molecular Formula	C46H51N4O9P
Molecular Weight	834.90 g/mol
Synonyms	5-Carbomethoxyvinyl-dU Phosphoramidite; 5-(E)-CMV-dU Phosphoramidite; 5-(E)-2-(Methoxycarbonyl)vinyl-2'-deoxyuridine 3'-phosphoramidite; DMT-CMV-dU-CE Phosphoramidite; 5-(trans-2-Carbomethoxyvinyl)-2'-deoxyuridine 3'-(2-cyanoethyl N,N-diisopropyl)phosphoramidite; 5-CMV-dU Phosphoramidite; 5-(E)-Carbomethoxyvinyldeoxyuridine Phosphoramidite
EINECS	Contact for details

Quality Control

Our 5-(E-2-Carbomethoxyvinyl)-5'-o-(4,4'-Dimethoxytrityl)-2'-Deoxyuridine, 3'-[(2-Cyanoethyl)-(N,n-Diisopropyl)]Phosphoramidite is manufactured under strict quality systems to ensure consistency and reliability for critical oligonucleotide synthesis. Each batch is subjected to rigorous analytical testing, including HPLC, 31P NMR, and 1H NMR, to verify identity, purity, and suitability for automated synthesis. Certificates of Analysis (COA) with full chromatographic data are provided to support your quality and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a desiccated environment. This compound is hygroscopic (moisture-sensitive) and easily oxidized (store under inert atmosphere). Allow the sealed vial to equilibrate to room temperature before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (31P NMR)	Conforms to reference standard
Purity (HPLC)	≥ 97.0%
Moisture Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.