Description

5'-O-(4,4'-Dimethoxytrityl)-5-Fluoro-O4-(2,4,6-Trimethylphenyl)-2'-Deoxyuridine, 3'-[(2-Cyanoethyl)-(N,N-Diisopropyl)]Phosphoramidite is a high-purity, specialty phosphoramidite monomer essential for the solid-phase synthesis of oligonucleotides. This compound is specifically engineered for the precise and efficient incorporation of modified nucleosides into DNA strands, enabling advanced research and therapeutic development. It is a critical raw material for pharmaceutical R&D, biotechnology companies, and diagnostic manufacturers focused on antisense oligonucleotides, siRNA, and other nucleic acid-based technologies.

Application

• Solid-Phase Oligonucleotide Synthesis: Primary monomer for automated synthesis of DNA strands containing 5-fluorouracil or other modified bases.
• Antisense Oligonucleotide (ASO) Development: Key building block for creating therapeutic ASOs targeting specific mRNA sequences.
• siRNA and miRNA Synthesis: Used in the manufacture of short interfering RNA (siRNA) and microRNA (miRNA) for gene silencing applications.
• Diagnostic Probe Manufacture: Essential for producing labeled DNA probes used in PCR, FISH, and microarray technologies.
• Pharmaceutical Research: Enables the development of novel nucleic acid-based drugs and aptamers.
• Academic & Biotech R&D: Supports fundamental research in molecular biology, genetics, and synthetic biology.

Basic Information

Item	Details
Product Name	5'-O-(4,4'-Dimethoxytrityl)-5-Fluoro-O4-(2,4,6-Trimethylphenyl)-2'-Deoxyuridine, 3'-[(2-Cyanoethyl)-(N,N-Diisopropyl)]Phosphoramidite
CAS No.	198080-36-3
Molecular Formula	C51H58FN4O8P
Molecular Weight	889.01 g/mol
Synonyms	5-Fluoro-2'-deoxyuridine, 5'-O-[bis(4-methoxyphenyl)phenylmethyl]-O4-(2,4,6-trimethylphenyl)-3'-[[[2-cyanoethyl)bis(1-methylethyl)phosphoramidite]; 5'-DMT-5-Fluoro-O4-(2,4,6-Trimethylphenyl)-2'-deoxyuridine-3'-CE Phosphoramidite; 5-Fluoro-dU(Mes) Phosphoramidite; 5-F-dU(Mes) Phosphoramidite; dU(F,Mes) Phosphoramidite; N/A (EINECS)

Quality Control

Our 5'-O-(4,4'-Dimethoxytrityl)-5-Fluoro-O4-(2,4,6-Trimethylphenyl)-2'-Deoxyuridine, 3'-[(2-Cyanoethyl)-(N,N-Diisopropyl)]Phosphoramidite is manufactured under strict quality systems to ensure the high purity and consistency required for sensitive oligonucleotide synthesis. Each batch undergoes rigorous analytical testing, including HPLC, 31P NMR, and 1H NMR, to verify identity, purity, and phosphoramidite functionality. Certificates of Analysis (COA) with full specification data are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a desiccated environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (31P NMR)	Conforms to reference standard
Purity (HPLC)	≥ 97.0%
Water Content (KF)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.