Description

Biotin Phosphoramidite For Abi is a high-purity, specialty phosphoramidite monomer designed for the automated solid-phase synthesis of oligonucleotides. This compound is critical for the site-specific introduction of biotin, a key molecule for labeling and detection, into synthetic DNA or RNA strands. It is an essential reagent for molecular biology researchers, diagnostic developers, and pharmaceutical companies engaged in probe development, next-generation sequencing, and therapeutic oligonucleotide manufacturing.

Application

• Oligonucleotide Synthesis: Primary building block for incorporating biotin tags during automated solid-phase DNA/RNA synthesis.
• Diagnostic Probe Development: Creation of biotinylated probes for use in hybridization assays, microarrays, and in-situ hybridization (FISH).
• Next-Generation Sequencing (NGS): Preparation of biotin-labeled capture probes for target enrichment and library preparation workflows.
• Therapeutic Oligonucleotides: Research and development of biotin-conjugated antisense oligonucleotides, siRNAs, and aptamers.
• Affinity Purification: Synthesis of oligonucleotides used for pull-down assays and streptavidin-based purification systems.
• Biosensor Fabrication: Immobilization of DNA strands on sensor surfaces via biotin-streptavidin interactions.

Basic Information

Product Name	Biotin Phosphoramidite For Abi
CAS No.	196408-65-8
Molecular Formula	C44H64N7O8P
Molecular Weight	850.00 g/mol
Synonyms	5'-Dimethoxytrityl-N-biotinyl-3'-aminopropyl-2'-deoxyadenosine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; Biotin-dA Phosphoramidite; Biotinylated dA CE Phosphoramidite; N-Biotin-5'-Dimethoxytrityl-3'-amino-2',3'-dideoxyadenosine, 2-Cyanoethyl N,N-Diisopropylphosphoramidite; Biotin Amidite; 5'-DMT-N-Biotin-dA-CE Phosphoramidite
EINECS	Contact for details

Quality Control

Our Biotin Phosphoramidite For Abi is manufactured under strict quality control protocols to ensure the high purity and consistency required for sensitive oligonucleotide synthesis. Each batch is analyzed by HPLC, NMR, and mass spectrometry. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and moisture content is provided with every shipment to support your quality assurance and regulatory documentation.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a desiccated environment. This product is hygroscopic (moisture-sensitive) and easily oxidized; prolonged exposure to air, moisture, or elevated temperatures will lead to degradation. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 97.0%
Water Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.