Description

Cytidine, n-Benzoyl-5'-o-[Bis(4-Methoxyphenyl)Phenylmethyl]-5-[(2-Cyanoethoxy)Methyl]-2'-Deoxy-, 3'-[2-Cyanoethyl N,n-Bis(1-Methylethyl)Phosphoramidite] is a sophisticated phosphoramidite monomer, a critical building block in the automated solid-phase synthesis of oligonucleotides. This compound matters because it enables the precise and scalable production of DNA sequences, which is foundational for modern genetic research, diagnostics, and therapeutic development. It is essential for biotechnology companies, pharmaceutical R&D departments, and academic research institutions engaged in genomics, synthetic biology, and the development of antisense oligonucleotides, siRNAs, and aptamers.

Application

• Oligonucleotide Synthesis: As a key phosphoramidite monomer for the automated chemical synthesis of DNA strands.
• Therapeutic Development: Production of antisense oligonucleotides (ASOs), small interfering RNAs (siRNAs), and aptamers for targeted therapies.
• Diagnostic Probes: Manufacture of labeled and unlabeled DNA probes for PCR, microarrays, and fluorescence in situ hybridization (FISH).
• Genomic Research: Synthesis of primers, linkers, and gene fragments for cloning, sequencing, and mutagenesis studies.
• Synthetic Biology: Construction of synthetic genes and genetic circuits for engineered biological systems.
• CRISPR/Cas9 Systems: Synthesis of guide RNA (gRNA) components and donor DNA templates for gene editing applications.

Basic Information

Product Name	Cytidine, n-Benzoyl-5'-o-[Bis(4-Methoxyphenyl)Phenylmethyl]-5-[(2-Cyanoethoxy)Methyl]-2'-Deoxy-, 3'-[2-Cyanoethyl N,n-Bis(1-Methylethyl)Phosphoramidite]
CAS No.	188411-06-5
Molecular Formula	C55H61N6O9P
Molecular Weight	981.09 g/mol
Synonyms	5'-O-DMT-5-(2-Cyanoethoxymethyl)-2'-deoxycytidine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; dC(MMT) Phosphoramidite; 5-Methyl-dC Phosphoramidite (MMT protected); 5-Methyl-2'-deoxycytidine-3'-phosphoramidite, MMT-protected; N-Benzoyl-5'-O-(4,4'-dimethoxytrityl)-5-[(2-cyanoethoxy)methyl]-2'-deoxycytidine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; 5-Methyl-dC(MMT) CE Phosphoramidite; 5-Methylcytidine phosphoramidite monomer
EINECS	Contact for details

Quality Control

This high-purity phosphoramidite monomer is manufactured under strict quality control protocols to ensure batch-to-batch consistency and optimal performance in automated synthesizers. Quality is verified through comprehensive analytical methods including HPLC, 31P NMR, and 1H NMR to confirm identity, purity, and the absence of critical impurities. A detailed Certificate of Analysis (COA) is provided with each batch, documenting specifications for assay, moisture content, and solvent residues to meet the exacting standards of pharmaceutical and diagnostic applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a desiccated environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation and moisture ingress.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms
Identification (31P NMR)	Conforms
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Solvent Residues (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.