Description

5-(n-[n-Biotinyl-ω-Aminocaproyl-γ-Aminobutyryl]-3-Aminoallyl)Uridine 5′-Triphosphate is a biotin-labeled nucleotide analog designed for sensitive detection and isolation of nucleic acids. This compound matters because it enables high-affinity, non-radioactive labeling for applications in molecular biology and diagnostics, offering superior specificity and stability. It is primarily needed by researchers and manufacturers in the fields of next-generation sequencing (NGS), microarray fabrication, and in situ hybridization.

Application

• Next-Generation Sequencing (NGS): Used as a labeled nucleotide for library preparation and target enrichment protocols.
• Microarray Technology: Essential for the synthesis of biotinylated probes for gene expression and genotyping arrays.
• Fluorescent In Situ Hybridization (FISH): Serves as a substrate for enzymatic labeling to generate high-sensitivity FISH probes.
• Pull-Down Assays: Enables the streptavidin-based capture and purification of specific DNA or RNA sequences.
• Diagnostic Kit Manufacturing: A key raw material for developing clinical diagnostic assays, including PCR-based tests.
• Single-Molecule Detection: Facilitates the labeling of nucleic acids for advanced microscopy and biosensing applications.

Basic Information

Item	Details
Product Name	5-(n-[n-Biotinyl-ω-Aminocaproyl-γ-Aminobutyryl]-3-Aminoallyl)Uridine 5′-Triphosphate
CAS No.	186033-13-6
Molecular Formula	C₃₄H₅₄N₉O₂₀P₃S
Molecular Weight	1059.89 g/mol
Synonyms	Biotin-16-aminoallyl-UTP; Biotin-16-aa-UTP; 5-(N-(6-(Biotinamido)hexyl)-3-aminoallyl)uridine-5'-triphosphate; Aminoallyl-biotin-UTP; Biotinylated aaUTP; Biotin-AA-UTP; 5-(3-Aminoallyl)-UTP-biotin conjugate; Biotin-XX-UTP (common trade name style)
EINECS	Contact for details

Quality Control

Our 5-(n-[n-Biotinyl-ω-Aminocaproyl-γ-Aminobutyryl]-3-Aminoallyl)Uridine 5′-Triphosphate is produced under stringent conditions suitable for molecular biology and in vitro diagnostic (IVD) use. Each batch undergoes rigorous quality testing, including HPLC for purity and functional assays to confirm labeling efficiency. Certificates of Analysis (COA) with detailed specifications are provided to ensure traceability and batch-to-batch consistency for your critical applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a non-frost-free freezer. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature before opening to minimize moisture uptake. For long-term storage, consider desiccant.

Specification

Item	Specification
Appearance	White to off-white solid or lyophilized powder
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 95%
Assay (UV)	≥ 90% (nucleotide basis)
Water Content (KF)	≤ 5.0%
Functional Activity	Conforms in enzymatic incorporation test

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.